Chemical name: Xylitol
Alias:Eutrit
Cas No.: 87-99-0
EINECS No.: 201-788-0
Chemical Formula: C5H12O5
Appearance: White crystalline powder
Grade: Food Grade
Shelf Life:  24 Months
Certification: ISO9001/Kosher/Halal/GMP
Application: Food additives- sweetener
Supply Ability: 10000 Ton/ per Year
MOQ:10000 kgs

Usage of Xylitol
1. To produce gum, chewing gum, toffee, soft candy, jelly, chocolate, chewing tablet and etc, can cool the throat, clean the teeth and be anti-cariogenic.
2. Being instead of sucrose to add in soft drink, milk, bread, preserved fruit, biscuit, yoghurt, jam, porridge and etc, to keep
longer & better sweet taste because of its non-fermentability by yeast.
3. Added in cosmetic product and toothpaste, no sticky feeling and refreshing. Xylitol can keep moisture and improve the rough skin similar as glycerin.
4. Being good substitute to sucrose, to make dishes like sour & sweet pork or fish, very safe to diabetics. Our suggestion is no more than 40g / day for adult and half for child.

 

In recent ten years,We are constantly pursuing good service of supplying high quality of the product and  competitive price.Our customers come from USA,India,Pakistan,Malaysia  and some African countries.We have accumulated much import and export experience.
With our continuous efforts,we can provide the following series of certificates.If you have other needs, please let us know.

 

 

Packed in 25kg/bag,1000kg/bag, Or Customized Package.
18MT in 20'FCL.

 

Tianjin Hwashin Biotechnology Co., Ltd is located in the Tianjin Jinbin industrial park, which is 30km to Beijing Daxing International Airport, 70km to Tianjin Binhai International Airport, and 100km to the Xingang port.
Since 2004, We have devoted ourselves to the research & development, production and sales of food additives, feed additives products. Currently, we have 4 business departments: Food Additives department, Feed additives department, Cosmetics materials department, Industrial and Daily Chemicals department. We have 4 productions bases in Tianjin, Hebei provice, Shandong provice and Jiangsu provice. Besides, we worked with the leading Chinese biotechnical and chemical producers, we offer more than 200 products to over 10 countries in the world. 
Hwashin team have excellent knowledge of biotechnology, chemicals and international business. Relied on a strict quality control system, through years of continuous technical innovation, the company could provide customers with first-class products and services.

1.To supply high-quality products with reasonable prices in food  additive industry.
2.Research on the Market Knowledge & trend follow up and inform customers of price trends in the market timely.Timely Deliver & Stock Promotion on hot demanding products.
3.To follow orders and arrange shipping in time, providing complete documents for Customs clearance according to different export policies in different countries.
4.Reliable & Strictly follow the contract responsibility & after sales service
5.Professional on International Logistic Service, Legalization documents & Third Party Inspection process.

Our Aim is that the goods reach the customers quickly and safely.

With the spread of the epidemic abroad,the shipping market has been greatly affected.
Nomally, most suppliers can't rent a boat and book a space.
However,it not difficult for us to solve the problem.
We have joined the local freight forwarding alliance.
It help us to get the accurate shipping information and solve the shipping problem.
And then we can help customers to make accurate decisions.

1. What are your payment terms?
Usually L/C,D/P,T/T, western union, moneygram or paypal.

2.How can we guarantee quality?
Always a pre-production sample before mass production;
Always final Inspection before shipment;

3. What's your delivery time?
lt depends on order quantity, usually we will arrange shipment within 3-20 days.

4.When can I get reply?
our sales manager will contact you within 12 hours.

5. How about the validity of products?
According to the products you order, usually 2 years.

6. What documents do you provide?
Usually, we provide Commercial nvoice, Packing List, Bill of Loading and OriginCertificate,etc.
lf there are any special requirements in your market, please let us know.

7. Can l get sample before order?
Yes, we can provide free sample once price is agreed, and you have to pay us the delivery fee in advance.If place following order after geting free sample, we will refund the shipping cost of free sample.

8.How to start orders or make payments?
A: You can send us your Purchase order, or just send a simple confirmation by email or by Trade Manager, after communication then we will send you Proforma Invoice with our bank details for final confirmation, then you can make payment accordingly.
 

 

1. Materials

Corncob: corncob is crushed, screened through 40 mesh, dried and preserved; Cellulase,

Candida species preservation medium: yeast extract 2.0g, peptone 3.0g

20G agar powder, 40g xylose, fixed volume at 1.0L, sterilized at 121 ºC for 20min

Liquid culture medium for strains: 10g glucose, 10g xylose, 2.0g yeast extract

Peptone 3.0g, wort 3.0g, constant volume at 1.0L, and at pH 5.0

Sterilization at 121 ºC for 20 min.

Fermentation medium: D-xylose 4%, peptone 0.15%, yeast powder 0.15%,

Ph6115 ºC autoclave for 20 min.

2. Method

2.1 preparation of corncob hydrolysate

Preparation of corncob hydrolysate: weigh 5.0g corncob powder and test at 30 U / g

Cellulase was added to the sample and adjusted to the solid-liquid ratio of 15 :1 with distilled water

After reacting in a 50 ºC water bath oscillator for 24 hours, the hydrolysate was centrifuged at 6000 R / min

For 15min, the supernatant was concentrated in vacuum at 70 ºC, and the concentrated solution was adjusted to 100 ºC with solid NaOH

Ph10.06000r/min centrifugation for 20 min to remove insoluble substances, and the supernatant was concentrated with

Sulfuric acid is adjusted back to pH5.5.

2.2 preparation of xylitol:

Seed culture: Take 1 ring of inclined bacteria, put it into seed culture medium, 250 ml

The volume of liquid in triangular bottle was 30ml, cultured in shake flask at 30 ºC for 48h, rotating speed was 150 R / min.

Shake flask fermentation: different fermentation environments are adopted according to the specific optimization conditions

Different fermentation strips such as fermentation temperature, pH, inoculation amount and liquid loading are commonly used

Add a certain amount of fermentation medium into a 250 ml shake flask to seed the liquid

The seeds were inoculated into the fermentation medium with a certain amount of inoculation. Bottle shaking speed 180

R / min, fermentation at 30 ºC for 72 h, sampling at fixed time to determine the yield of xylitol.

 2.3 xylitol content detection

For xylitol content detection, take 0.5 ml of appropriately diluted sample and add periodate reagent

0.5ml, mix well, add 5ml of chromotropic acid reagent, shake, boiling water bath for 30 minutes

Min, cooled, photometric absorption value at 570 nm wavelength, determined by under the same conditions

The measured standard sample is obtained by linear equation.

Xylitol yield (%) = amount of xylitol obtained by fermentation × 100%/Theoretical value of total xylitol obtained by complete fermentation of raw

materials.