Product Introduction

DMEM/F-12 medium is modified by mixing DMEM and Ham's F-12 medium at a ratio of 1:1. The modified DMEM/F-12 medium is richer in nutrients and contains more trace elements. It is widely used in the cultivation of a variety of mammalian cells, and also suitable for the cultivation and cloning of mammalian cells with low serum content. Density cultivation. DMEM/F-12 is also used as a basic medium for the development of serum-free medium.

This product contains amino acids, vitamins, inorganic salts and other ingredients required for cell culture. It does not contain protein or growth factors. It is necessary to add 5-10% serum or serum-free additives according to the cell type.

This product is sterilized by filtration through a 0.1 μm filter membrane, pH 7.0-7.4, contains 17.5 mM D-glucose, added 2.5 mM L-glutamine, 0.5 mM sodium BT, contains phInol red indicator, and does not contain HEPES buffer system.


Storage and transportation

Transport in wet ice; store at 2-8°C, valid for 12 months.


Precautions

1. This product has been filtered and sterilized. When using it, you should pay attention to aseptic operation to avoid contamination;

2. In order to maintain the best results, please do not freeze-thaw.