Dmem + Ham′ S F-12 Medium

China Dmem + Ham′ S F-12 Medium, Find details about China Serum-Free Medium, Antibody Medium from Dmem + Ham′ S F-12 Medium

Model NO.
G4613-500ML
Usage
Laboratory Reagents, Analytical Reagents, Teaching Reagents
Habit Appellation
Special Reagent
Application
Industry, Scientific Research, Health, Environmental Protection
Property
Organic Reagent
Trademark
Servicebio
Specification
500ML
Origin
China
Model NO.
G4613-500ML
Usage
Laboratory Reagents, Analytical Reagents, Teaching Reagents
Habit Appellation
Special Reagent
Application
Industry, Scientific Research, Health, Environmental Protection
Property
Organic Reagent
Trademark
Servicebio
Specification
500ML
Origin
China
Product Introduction

The DMEM/F-12 medium is modified by mixing DMEM and Ham' F-12 medium at a ratio of 1:1. The modified DMEM/F-12 medium is richer in nutrients and contains more trace elements. It is widely used in the cultivation of a variety of mammalian cells, and also suitable for the cultivation and cloning of mammalian cells with low serum content. Density cultivation. DMEM/F-12 is also used as a basic medium for the development of serum-free medium. This product contains amino acids, vitamins, inorganic salts and other ingredients required for cell culture. It does not contain protein or growth factors. It is necessary to add 5-10% serum or serum-free additives according to the cell type.

This product is sterilized by 0.1 μm filter membrane, pH 7.0-7.4, containing 17.5 mM D-glucose, adding 2.5 mM L-glutamine, 0.5 mM sodium BT, containing pheol red indicator, containing 15 mM HEPES buffer system.

HEPES is a commonly used biological buffer, which can keep the culture medium in a constant pH range for a long time and effectively prevent large fluctuations in the pH of the culture fluid from adversely affecting cell growth.


Storage and transportation

Transport in wet ice; store at 2-8°C, valid for 12 months.


Precautions

1. This product has been filtered and sterilized. When using, pay attention to aseptic operation to avoid contamination;

2. In order to maintain the best results, please do not freeze-thaw.


Dmem + Ham′ S F-12 MediumDmem + Ham′ S F-12 MediumDmem + Ham′ S F-12 Medium