Sealed serum is used to seal samples in immunohistochemistry or immunofluorescence experiments. In the selection of blocking serum, it should be noted that the blocking serum should not contain the target protein,And different from the primary antibody source, the same source of blocked serum can be used with the secondary antibody.Closed serums are suitable for immunosistification or immunofluorescence experiments where samples are closed. The sites that bind to protein nonspecificity in the sample to be tested can be closed, and the endogenous Fc fragment binding sites in the sample can also be closed, blocking the binding of antibodies to Fc receptors in the sample, thereby reducing the background and false positives. In choosing a closed serum, it is important to note that the closed serum cannot contain the target protein, and unlike one anti-source, a closed serum from the same source as a second-resistance can be used.
Introduction for use
When used, dilute the closed serum 10 or 20 times with PBS (0.01 mol/L, pH7.2-7.4) with 10 or 5%The working fluid, directly drips added to tissue slices or cell crucibles, incubate at 37 degrees C for 10-30 minutes, remove serum, do not wash, drip fit When the ratio of diluted anti-working fluid, incubate at 37 degrees C 1-2 h or 4 degrees C overnight.

Company information

Founded in 2004, Beijing Solarbio Science & technology Co. Ltd. is a large high-tech biological enterprise integrating R & D, production, sales and service. Headquartered in Beijing, the company has branches or agents in more than 30 provinces and cities across the country.

With a nationwide service network, it is one of the largest life science R & D Reagent Suppliers in China. Our company exports products to American, Japan and other developed countries in Europe. Through the Alibaba international website and has been highly recognized in the international market.

At present, our company supplies nearly 10,000 kinds of products, over 5,000 of which are available in stock. Our products involve molecular biology, cell biology, immunology, biomedicine and other relevant areas. We continuously expand the range of our products to provide customers with the best and most professional products.

Our products have been widely used in many famous scientific research institutes such as China Academy of Sciences, Tsinghua University, Beijing University and Fudan University. With reliable and stable quality product and perfect after-sale service we established a good brand image. Now Solarbio has been recognized by scientific researchers in China and become a famous band recommended by many distributors.

With professional R & D and quality inspection team, advanced storage and logistics system as well as strict GMP and ISO 9000 standards for production and management, we are committed to provide users with the best reagents, the most professional products and the most perfected solutions, aiming to build a world-class national brand.

We are determined to take an independent brand strategy. Meanwhile, we look forward to further cooperation with more scientific research institutes and make efforts for the development of human life science research to achieve mutual benefit and win-win results.