Triton X-100 Reagent Grade 100ml 500ml
China Triton X-100 Reagent Grade 100ml 500ml, Find details about China Triton, Triton X-100 from Triton X-100 Reagent Grade 100ml 500ml
Basic Info.
Model NO.
T8200
Trademark
Solarbio
Specification
100ml/500ml
Origin
Beijing, China
Model NO.
T8200
Trademark
Solarbio
Specification
100ml/500ml
Origin
Beijing, China
Product Desciption
Triton X-100
Product Description
Appearance: Light colored viscous
Molecular Weight: 647
Triton X-100 is a nonionic detergent, 100% active ingredient, which is often used in biochemical applications to solubilize proteins. Triton X-100 has no antimicrobial properties. It is considered a comparatively mild non-denaturing detergent and is reported in numerous references. It does absorb in the ultraviolet region of the spectrum, so it can interfere with protein quantitation by absorption at A280nm. A number of polymeric resins have been used to remove X-100 from solution, including Amberlite hydrophobic XAD resins and Rezorian A161 cartridges. For lysing cells, typically about 0.1% Triton X-100 solution in water will be sufficient, and even up to 0.5% concentrations will usually not harm most enzymes being isolated. Many enzymes remain active in the presence of X-100; for example, Proteinase K, remains active in 1% (w/w) solutions of X-100. Triton X-100 can be detected in the parts per million range by spectrophotometric measurement of the concentration of a Triton-ammonium-cobalt-thiocyanate complex. Interfering substances in this assay have been discussed. For a given application, the choice of a suitable surfactant depends on a number of variables, from its solubility, polarity and micelle size to the mechanism of its action with the target solute. The literature contains numerous articles: Choice of detergent for solubilization of (erythrocyte) membranes; Effect of hydrophile-lipophile balance on (cytochrome) membrane solubilization; Mode of interaction of polyoxyethylene glycol detergents with membrane protein ; General background on surfactants and use in protein purification.
Company information
Founded in 2004, Beijing Solarbio Science & technology Co. Ltd. is a large high-tech biological enterprise integrating R & D, production, sales and service. Headquartered in Beijing, the company has branches or agents in more than 30 provinces and cities across the country.
With a nationwide service network, it is one of the largest life science R & D Reagent Suppliers in China. Our company exports products to American, Japan and other developed countries in Europe. Through the Alibaba international website and has been highly recognized in the international market.
At present, our company supplies nearly 10,000 kinds of products, over 5,000 of which are available in stock. Our products involve molecular biology, cell biology, immunology, biomedicine and other relevant areas. We continuously expand the range of our products to provide customers with the best and most professional products.
Our products have been widely used in many famous scientific research institutes such as China Academy of Sciences, Tsinghua University, Beijing University and Fudan University. With reliable and stable quality product and perfect after-sale service we established a good brand image. Now Solarbio has been recognized by scientific researchers in China and become a famous band recommended by many distributors.
With professional R & D and quality inspection team, advanced storage and logistics system as well as strict GMP and ISO 9000 standards for production and management, we are committed to provide users with the best reagents, the most professional products and the most perfected solutions, aiming to build a world-class national brand.
We are determined to take an independent brand strategy. Meanwhile, we look forward to further cooperation with more scientific research institutes and make efforts for the development of human life science research to achieve mutual benefit and win-win results.
Product Description
Appearance: Light colored viscous
Molecular Weight: 647
Triton X-100 is a nonionic detergent, 100% active ingredient, which is often used in biochemical applications to solubilize proteins. Triton X-100 has no antimicrobial properties. It is considered a comparatively mild non-denaturing detergent and is reported in numerous references. It does absorb in the ultraviolet region of the spectrum, so it can interfere with protein quantitation by absorption at A280nm. A number of polymeric resins have been used to remove X-100 from solution, including Amberlite hydrophobic XAD resins and Rezorian A161 cartridges. For lysing cells, typically about 0.1% Triton X-100 solution in water will be sufficient, and even up to 0.5% concentrations will usually not harm most enzymes being isolated. Many enzymes remain active in the presence of X-100; for example, Proteinase K, remains active in 1% (w/w) solutions of X-100. Triton X-100 can be detected in the parts per million range by spectrophotometric measurement of the concentration of a Triton-ammonium-cobalt-thiocyanate complex. Interfering substances in this assay have been discussed. For a given application, the choice of a suitable surfactant depends on a number of variables, from its solubility, polarity and micelle size to the mechanism of its action with the target solute. The literature contains numerous articles: Choice of detergent for solubilization of (erythrocyte) membranes; Effect of hydrophile-lipophile balance on (cytochrome) membrane solubilization; Mode of interaction of polyoxyethylene glycol detergents with membrane protein ; General background on surfactants and use in protein purification.
Founded in 2004, Beijing Solarbio Science & technology Co. Ltd. is a large high-tech biological enterprise integrating R & D, production, sales and service. Headquartered in Beijing, the company has branches or agents in more than 30 provinces and cities across the country.
With a nationwide service network, it is one of the largest life science R & D Reagent Suppliers in China. Our company exports products to American, Japan and other developed countries in Europe. Through the Alibaba international website and has been highly recognized in the international market.
At present, our company supplies nearly 10,000 kinds of products, over 5,000 of which are available in stock. Our products involve molecular biology, cell biology, immunology, biomedicine and other relevant areas. We continuously expand the range of our products to provide customers with the best and most professional products.
Our products have been widely used in many famous scientific research institutes such as China Academy of Sciences, Tsinghua University, Beijing University and Fudan University. With reliable and stable quality product and perfect after-sale service we established a good brand image. Now Solarbio has been recognized by scientific researchers in China and become a famous band recommended by many distributors.
With professional R & D and quality inspection team, advanced storage and logistics system as well as strict GMP and ISO 9000 standards for production and management, we are committed to provide users with the best reagents, the most professional products and the most perfected solutions, aiming to build a world-class national brand.
We are determined to take an independent brand strategy. Meanwhile, we look forward to further cooperation with more scientific research institutes and make efforts for the development of human life science research to achieve mutual benefit and win-win results.
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