High Purity Low Electroosmotic Agarose for Nucleic Acid Electrophoresis
China High Purity Low Electroosmotic Agarose for Nucleic Acid Electrophoresis, Find details about China Agarose, Nucleic Acid Electrophoresis from High Purity Low Electroosmotic Agarose for Nucleic Acid Electrophoresis
Basic Info.
Model NO.
G5056-100G
Property
Biochemical Reagent
Trademark
Servicebio
Specification
100G
Origin
China
Model NO.
G5056-100G
Property
Biochemical Reagent
Trademark
Servicebio
Specification
100G
Origin
China
Product Desciption
Product introduction:
Agarose is a linear polysaccharide composed of two monosaccharides, galactose and endoether galactose, alternately linked. Agarose is a white or yellowish powder with no odor. Hot water is the best solvent. It is also soluble in dimethyl sulfoxide and formamide solutions. Agarose is generally heated to more than 90ºC in water to dissolve, when the temperature drops to 35-40ºC to form a good semi-solid gel, which is the main feature and basis of its many uses.As the agarose solution cools, the sugar chains of the two agarose molecules intertwine in a double helix pattern, creating a three-dimensional network. After further cooling, the discrete double helices would come together and pack together to form a harder gel.It is often used to prepare nucleic acid analysis gels, such as agarose gels for DNA or RNA electrophoresis.
Package Contents:
Storage condition:
Stored at room temperature and dry, valid for 24 months.
Product Features:
Usage:
1. Prepare an appropriate amount of glue preparation and electrophoretic buffer (usually 0.5 x TBE or 1 x TAE buffer) (G3001 and G3002 are recommended);
2. Add the accurately weighed AGAR powder into the glass conical flask containing a certain amount of electrophoretic buffer according to the amount of glue and gel concentration (the volume of the added buffer should not exceed 50% of the conical flask capacity);
3. Use plastic wrap or PE gloves to cover the mouth of the conical bottle, and then heat and dissolve the agarose in the microwave oven (Note: When the solution is boiling during heating, please wear insulating gloves and carefully rock the conical bottle so that the agarose is fully and evenly dissolved. Repeat this operation several times until the agarose particles are completely dissolved;The heating time in the microwave oven should not be too long, each time when the solution begins to boil and bubble to stop heating, otherwise it is easy to cause the solution to overheat and boil out, resulting in inaccurate gel concentration;At the same time, when the agarose is dissolved, it must be completely dissolved, otherwise the electrophoresis image will be blurred or bright particles will appear);
4. When the solution is cooled to about 50ºC, a certain proportion of nucleic acid dye (Serred nucleic acid dye or other nucleic acid dye, etc.) can be added (G3606 is recommended) and fully mixed;
5. Pour the agarose solution into the gelatin-making tank, select the appropriate comb and insert it into the corresponding position. The gel thickness is generally controlled between 3 ~ 5mm.
6. The gel can solidify at room temperature for about 30 minutes (the solidification time of the gel varies with different concentrations, which can be adjusted according to the actual situation). After pulling out the comb, the gel is placed in the electrophoresis tank for electrophoresis, and the electrophoresis buffer should not pass through the sample adding hole in the gel.
Agarose gel concentration and linear DNA separation range
Notes:
1. The buffer used to prepare the gel should be exactly the same as the buffer used for electrophoresis.
2. After adding the nucleic acid dye, it should be fully mixed, otherwise it is easy to cause the distortion of the electrophoresis strip.
3. If high concentration (>=2.5%) agarose gel is needed, it can be left standing at room temperature for 10min after Step 3, and then heated agarose solution. This operation is conducive to more uniform dissolution of agarose.
4. Agarose gels are recommended for ready use, or at room temperature for no more than 4 hours.If the gel needs to be stored for a long time, the gel can be wrapped in plastic wrap and placed at 4ºC and protected from light. Generally, the gel can be stored for 2-5 days, and the brightness or clarity of the electrophoresis strip may be slightly reduced.
5. If the DNA in the cutting gel strips is recovered by the gel recovery kit, the melting temperature of the strips is recommended to be 60±5ºC according to the product instructions of the kit.
Agarose is a linear polysaccharide composed of two monosaccharides, galactose and endoether galactose, alternately linked. Agarose is a white or yellowish powder with no odor. Hot water is the best solvent. It is also soluble in dimethyl sulfoxide and formamide solutions. Agarose is generally heated to more than 90ºC in water to dissolve, when the temperature drops to 35-40ºC to form a good semi-solid gel, which is the main feature and basis of its many uses.As the agarose solution cools, the sugar chains of the two agarose molecules intertwine in a double helix pattern, creating a three-dimensional network. After further cooling, the discrete double helices would come together and pack together to form a harder gel.It is often used to prepare nucleic acid analysis gels, such as agarose gels for DNA or RNA electrophoresis.
Package Contents:
| Cat. No. | Product description | Volume |
| G5056 | High purity and low electroosmotic agarose | 5g/100g |
Storage condition:
Stored at room temperature and dry, valid for 24 months.
Product Features:
| CAS number | 9012-36-6 |
| Appearance | White to off-white powder |
| Gel intensity | ≥1200 g/cm2 (1% Gel) |
| Gelling temperature | 36±1.5ºC (1.5% Gel) |
| Melt glue temperature | 88±1.5ºC (1.5% Gel) |
| Electroseepage value | ≤0.13 |
| sulphate | ≤0.15% |
| Moisture | ≤10% |
| DNase | None Detected |
| RNase | None Detected |
| Protease | None Detected |
Usage:
1. Prepare an appropriate amount of glue preparation and electrophoretic buffer (usually 0.5 x TBE or 1 x TAE buffer) (G3001 and G3002 are recommended);
2. Add the accurately weighed AGAR powder into the glass conical flask containing a certain amount of electrophoretic buffer according to the amount of glue and gel concentration (the volume of the added buffer should not exceed 50% of the conical flask capacity);
3. Use plastic wrap or PE gloves to cover the mouth of the conical bottle, and then heat and dissolve the agarose in the microwave oven (Note: When the solution is boiling during heating, please wear insulating gloves and carefully rock the conical bottle so that the agarose is fully and evenly dissolved. Repeat this operation several times until the agarose particles are completely dissolved;The heating time in the microwave oven should not be too long, each time when the solution begins to boil and bubble to stop heating, otherwise it is easy to cause the solution to overheat and boil out, resulting in inaccurate gel concentration;At the same time, when the agarose is dissolved, it must be completely dissolved, otherwise the electrophoresis image will be blurred or bright particles will appear);
4. When the solution is cooled to about 50ºC, a certain proportion of nucleic acid dye (Serred nucleic acid dye or other nucleic acid dye, etc.) can be added (G3606 is recommended) and fully mixed;
5. Pour the agarose solution into the gelatin-making tank, select the appropriate comb and insert it into the corresponding position. The gel thickness is generally controlled between 3 ~ 5mm.
6. The gel can solidify at room temperature for about 30 minutes (the solidification time of the gel varies with different concentrations, which can be adjusted according to the actual situation). After pulling out the comb, the gel is placed in the electrophoresis tank for electrophoresis, and the electrophoresis buffer should not pass through the sample adding hole in the gel.
Agarose gel concentration and linear DNA separation range
| Agarose gel concentration | Linear DNA separation range (bp) |
| 0.5% | 1000 ~ 30000 |
| 0.7% | 800 ~ 12000 |
| 1.0% | 500 ~ 10000 |
| 1.2% | 400 ~ 7000 |
| 1.5% | 200 ~ 3000 |
| 2.0% | 50 ~ 2000 |
Notes:
1. The buffer used to prepare the gel should be exactly the same as the buffer used for electrophoresis.
2. After adding the nucleic acid dye, it should be fully mixed, otherwise it is easy to cause the distortion of the electrophoresis strip.
3. If high concentration (>=2.5%) agarose gel is needed, it can be left standing at room temperature for 10min after Step 3, and then heated agarose solution. This operation is conducive to more uniform dissolution of agarose.
4. Agarose gels are recommended for ready use, or at room temperature for no more than 4 hours.If the gel needs to be stored for a long time, the gel can be wrapped in plastic wrap and placed at 4ºC and protected from light. Generally, the gel can be stored for 2-5 days, and the brightness or clarity of the electrophoresis strip may be slightly reduced.
5. If the DNA in the cutting gel strips is recovered by the gel recovery kit, the melting temperature of the strips is recommended to be 60±5ºC according to the product instructions of the kit.
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