Reagent

Quantity

Storage

1

Assay plate    

1

Stored at -20°C

2

Standard

2

3

Sample Diluent

1 × 20ml

4

Assay Diluent A

1 × 10ml

5

Assay Diluent B

1 × 10ml

6

Detection Reagent A

1 × 120μl

7

Detection Reagent B

1 × 120μl

8

Wash Buffer(25 x concentrate)

1 × 30ml

Stored at 4°C

9

Substrate

1 × 10ml

10

Stop Solution

1 × 10ml

11

Plate sealer for 96 wells

5

R.T.

12

Instruction

1

R.T.