Basic Info.

Model NO.

P1031

Model NO.

P1031

Product Desciption

Taq Plus DNA Polymerase
#P1031, 250U

Concentration: 2.5U/μl
Contents:

Taq Plus DNA Polymerase	100 μl
10× PCR Buffer (Mg2+ Plus)	1.25 ml
6× Loading Buffer	1 ml

Store at -20°C
For research use only.

Description
Taq Plus DNA Polymerase is a mixture of Taq and Pfu polymerase, blends the processivity of taq with the high fidelity of Pfu. The two enzymes act synergistically during PCR to generate more accurate and longer PCR products with greater yields compared to Taq DNA Polymerase alone. It can amplify DNA target up to 20 kb (simple template). And it is suitable as a direct replacement for ordinary Taq Polymerase in most applications. PCR products amplified by Taq Plus are mixture of blunt-ends and 3'dA-overhangs. The error rate of this PCR amplification is 1.0×10^-5 per nucleotide per cycle. 
Unit Definition
One unit is defined as the amount of the enzyme required to catalyze the incorporation of 10 nmole of dNTPs into an acid-insoluble form in 30 minutes at 70°C using hering sperm DNA as substrate.
Storage Buffer
20mM Tris-HCl (pH 8.0), 100mM KCl, 3mM MgCl_2, 1mM DTT, 0.1% NP-40, 0.1% Tween20, 0.2mg/ml BSA, 50% (V/V) glycerol.

Quality Control
The absence of endodeoxyribonucleases, exodeoxyribonucleases and ribonucleases is confirmed by appropriate quality tests. Functionally tested in amplification of a single-copy gene from human genomic DNA.
Endodeoxyribonuclease Assay
No detectable conversion of covalently closed circular DNA to a nicked DNA was observed after incubation of 10U Pfu DNA Polymerase with 1μg pBR322 DNA for 4 hours at 37°C  and 70°C.
Exodeoxyribonuclease Assay
No detectable degradation of lambda DNA-HindIII fragments was observed after incubation of 10U Pfu DNA Polymerase with 1μg digested DNA for 4 hours at 37°C and 70°C.
Ribonuclease Assay
0% of the total radioactivity was released into trichloroacetic acid-soluble fraction after incubation of 10U Pfu DNA Polymerase with 1μg E.coli [3H]-RNA (40000cpm/μg) for 4 hours at 37°C and 70°C.




Dongsheng Biotech offers different series of products to help you achieve PCR success. For enzymes, our Taq Polymerase, HS Taq DNA Polymerase, FS Taq DNA Polymerase, Pfu DNA Polymerase and Fusion Pfu DNA Polymerase provide high fidelity, efficient, sensitivity PCR performance. We also have Long Taq DNA Polymerase for long PCR performance.