Isopropyl β-D 1thiogalactopyranoside (IPTG) is a molecular biology reagent. This compound is a molecular mimic of allolactose, a lactose metabolite that triggers transcription of the lac operon, and it is therefore used to induce protein expression where the gene is under the control of the lac operator. IPTG, unlike allolactose, is not hydrolyzable by βgalactosidase, its concentration, therefore, remains constant in an experiment. For induction, a sterile, filtered 1 M solution of IPTG is typically added by 1:1000 dilution into a logarithmically growing bacterial culture, however, the different final concentration of IPTG may also be used.
 

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