Ponceau S Staining 100ml for Western Blot Immunoblotting
China Ponceau S Staining 100ml for Western Blot Immunoblotting, Find details about China Ponceau Red S, Fast Ponceau 2b from Ponceau S Staining 100ml for Western Blot Immunoblotting
Basic Info.
Model NO.
G2011-100ML
Application
Industry, Scientific Research
Trademark
Servicebio
Specification
100ML
Origin
China
Model NO.
G2011-100ML
Application
Industry, Scientific Research
Trademark
Servicebio
Specification
100ML
Origin
China
Product Desciption
Product informaiton
Product introduction
Ponceau S staining solution can be used for rapid detection of proteins on PVDF membrane, nitrcellulose membrane (NC membrane) and cellulose acetate membrane. The principle is that Ponceau S staining is negatively charged and can combine with positively charged amino acid residues. Meanwhile, Ponceau S staining can also combine with the non-polar region of the protein to form red bands. The protein staining is reversible and can be washed with distilled water, PBS or other appropriate solutions after staining.
The main ingredient of this product is 0.2% Ponceau S staining.
Storage and transportation
Store and transport at room temperature, validity for 12 months.
Specification
Operating methods
1. Immerse PVDF membrane, nitrocelllose membrane or cellulose acetate membrane in Ponceau S working solution, shake and stain for 3-5 min or longer till a clear band appeared.
2. Rinse the dyed film in distilled water for a few seconds, slightly remove the background color, until the background is light and the protein bands are clear.
3. Rinse with distilled water, PBS or other appropriate solutions for 2-3 times, 3-5min each time, remove Ponceau S, and conduct subsequent Western experiment.
Notes:
1. Ponceau S staining solution can be recycled repeatedly.
2. Ponceau S staining solution is not suitable for protein staining detection on nylon film.
3. For your safety and health, please wear lab coat and disposable gloves for operation.
| Product name | Cat. No. | Specification |
| Ponceau S Staining | G2011-100ML | 100 mL |
Product introduction
Ponceau S staining solution can be used for rapid detection of proteins on PVDF membrane, nitrcellulose membrane (NC membrane) and cellulose acetate membrane. The principle is that Ponceau S staining is negatively charged and can combine with positively charged amino acid residues. Meanwhile, Ponceau S staining can also combine with the non-polar region of the protein to form red bands. The protein staining is reversible and can be washed with distilled water, PBS or other appropriate solutions after staining.
The main ingredient of this product is 0.2% Ponceau S staining.
Storage and transportation
Store and transport at room temperature, validity for 12 months.
Specification
| Component | G2011-100ML |
| Ponceau S staining solution | 100 mL |
| Manual | 1 |
Operating methods
1. Immerse PVDF membrane, nitrocelllose membrane or cellulose acetate membrane in Ponceau S working solution, shake and stain for 3-5 min or longer till a clear band appeared.
2. Rinse the dyed film in distilled water for a few seconds, slightly remove the background color, until the background is light and the protein bands are clear.
3. Rinse with distilled water, PBS or other appropriate solutions for 2-3 times, 3-5min each time, remove Ponceau S, and conduct subsequent Western experiment.
Notes:
1. Ponceau S staining solution can be recycled repeatedly.
2. Ponceau S staining solution is not suitable for protein staining detection on nylon film.
3. For your safety and health, please wear lab coat and disposable gloves for operation.
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