Feulgen Stain Kit for DNA Gene Staining Reagent
China Feulgen Stain Kit for DNA Gene Staining Reagent, Find details about China Gene Staining Reagent, Feulgen Stain Kit from Feulgen Stain Kit for DNA Gene Staining Reagent
Basic Info.
Model NO.
G1048-50ML
Application
Industry, Scientific Research
Property
Biochemical Reagent
Trademark
Servicebio
Specification
50ML
Origin
China
Model NO.
G1048-50ML
Application
Industry, Scientific Research
Property
Biochemical Reagent
Trademark
Servicebio
Specification
50ML
Origin
China
Product Desciption
Principle
Feulgen stain is a staining technique used in histology to identify chromosomal material or DNA in cell specimens. It depends on acid hydrolysis of DNA in the 1-4 glycosidic bond, generating free aldehyde group. Then the aldehyde group combined with magenta forming purple-red complexes. DNA synthesis increase significantly during cell hyperplasia in pathological tissue (tumor, granulation). With Feulgen stain, the distribution (aggregate or disperse) and amount of DNA can be demonstrated.
Package content
Preservation
Valid in 6 months at 4ºC.
Procedure
1,Deparaffinize and hydrate sections to distilled water.
2,Preheat 1 M hydrocloric acid solution in 60ºC oven. Immerse slides in warm 1 M hydrocloric acid for 30 min, rinse in room-temperature1 M hydrocloric acid, wash with distilled water for 3 times.
3,immerse in perodic acid solution for 60-90 min, placed in dark condition. Do not wash in water.
4,Rinse slides in 2 changes of Sodium metabisulfite solution, each 2 min. Wash in running tap water for 5 min.
5,Rinse in fast-green solution for 10 s-2 min, dehydrate in 3 changes of absolute alcohol.
6,Clear in 2 changes of xylene, 5 min each. Mount with resin mounting medium.
7,Microscopy determination and collect images for analysis.
Results
Nucleus DNA is purple-red while cytoplasm and other elements are light green.
Negative control
Notes
1,Normal fix solution are alternative except Bouin solution which may result in over-hydrolysis of DNA. So Bouin solution is not suitable.
2,Too long or short time for hydrohloric acid hydrolysis both result in pale stain of nucleus. Generally, hydrolysis for 8 min is appropriate to most tissues. Adjusting the time according to hydrolysis result is recommended.
3,Manage the Fast-green counterstain in a proper range. Over-stain may cause deep blue or even overlap the positive results.
Feulgen stain is a staining technique used in histology to identify chromosomal material or DNA in cell specimens. It depends on acid hydrolysis of DNA in the 1-4 glycosidic bond, generating free aldehyde group. Then the aldehyde group combined with magenta forming purple-red complexes. DNA synthesis increase significantly during cell hyperplasia in pathological tissue (tumor, granulation). With Feulgen stain, the distribution (aggregate or disperse) and amount of DNA can be demonstrated.
Package content
| Code | Name | Size |
| G1048-1 | 1 M hydrocloric acid | 50ml |
| G1048-2 | Magenta solution | 50ml |
| G1048-3 | Sodium metabisulfite solution | 50ml |
| G1048-4 | Fast-green solution | 50ml |
Preservation
Valid in 6 months at 4ºC.
Procedure
1,Deparaffinize and hydrate sections to distilled water.
2,Preheat 1 M hydrocloric acid solution in 60ºC oven. Immerse slides in warm 1 M hydrocloric acid for 30 min, rinse in room-temperature1 M hydrocloric acid, wash with distilled water for 3 times.
3,immerse in perodic acid solution for 60-90 min, placed in dark condition. Do not wash in water.
4,Rinse slides in 2 changes of Sodium metabisulfite solution, each 2 min. Wash in running tap water for 5 min.
5,Rinse in fast-green solution for 10 s-2 min, dehydrate in 3 changes of absolute alcohol.
6,Clear in 2 changes of xylene, 5 min each. Mount with resin mounting medium.
7,Microscopy determination and collect images for analysis.
Results
Nucleus DNA is purple-red while cytoplasm and other elements are light green.
Negative control
- Replace the hydrohloric acid in step 7, 8, and 9 with distilled water.
- After deparaffinize, incubate slides with DNase (1 mg/ml) at 37ºC for 2 h.
Notes
1,Normal fix solution are alternative except Bouin solution which may result in over-hydrolysis of DNA. So Bouin solution is not suitable.
2,Too long or short time for hydrohloric acid hydrolysis both result in pale stain of nucleus. Generally, hydrolysis for 8 min is appropriate to most tissues. Adjusting the time according to hydrolysis result is recommended.
3,Manage the Fast-green counterstain in a proper range. Over-stain may cause deep blue or even overlap the positive results.
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