Hematoxylin Eosin Dyeing Reagent Suit for Tissues Cells Staining 500ml

China Hematoxylin Eosin Dyeing Reagent Suit for Tissues Cells Staining 500ml, Find details about China Hematoxylin-Eosin Staining Reagent, Hematoxylin Eosin Stain from Hematoxylin Eosin Dyeing Reagent Suit for Tissues Cells Staining 500ml

Model NO.
G1005-500ML
Habit Appellation
Chemical Medicine
Application
Industry, Scientific Research
Property
Biochemical Reagent
Trademark
Servicebio
Specification
500ML
Origin
China
Model NO.
G1005-500ML
Habit Appellation
Chemical Medicine
Application
Industry, Scientific Research
Property
Biochemical Reagent
Trademark
Servicebio
Specification
500ML
Origin
China
Product Introduction

HE staining, which is the combined staining of Hematoxylin and Eosin, is the most basic and most widely used technical method in histology, embryology, and pathology teaching and scientific research. Hematoxylin dye is alkaline, which mainly makes the chromatin in the nucleus and the nucleic acid in the cytoplasm colored blue to blue-violet; Eosin is an acid dye, which mainly makes the components in the cytoplasm and extracellular matrix colored red. HE staining can be used to observe and compare the morphology and structure of normal tissues and diseased tissues, and can preliminarily determine or identify abnormal substances in diseased tissues and cells. The nucleus of the tissues or cells stained with the HE stain of this product is blue to blue-purple, the cytoplasm is red, and the cytoplasm and nucleus are in sharp contrast.


Storage and transportation

Store and transport at room temperature. The validity period is 18 months.

Preparation before experiment

Bring your own hematoxylin differentiation solution (recommended G1039), hematoxylin re-blue solution (recommended G1040), xylene, gradient ethanol, absolute ethanol, neutral gum, etc.


Steps

1. Sample preparation

(1) For paraffin sections: sections are deparaffinized with xylene for 10 minutes, replaced with fresh xylene for 10 minutes, absolute ethanol for 5 minutes, fresh absolute ethanol for 5 minutes, 90% ethanol for 5 minutes, and 75% ethanol for 5 minutes. , Wash with tap water.

(2) For frozen sections: frozen sections stored at -20°C should be allowed to stand for 5-10 minutes to return to room temperature. If fixation is required, wash with tap water after the required fixative at room temperature.

2. HE staining

(1) Hematoxylin staining: the slices processed above are stained with hematoxylin staining solution for 3-5 min, and then washed with tap water;

(2) Differentiation: the slices are treated with hematoxylin differentiation solution for 2-5 s, and then rinsed thoroughly with running water;

(3) Return to blue: Hematoxylin blue liquid returns to blue for 2-5 s, rinse thoroughly with running water;

(4) Eosin staining: the slices were dehydrated by 85% and 95% gradient ethanol successively, each for 5 min. Then enter the eosin dye solution (alcohol-soluble) for dyeing for 5 minutes, and then dehydrate twice with anhydrous ethanol for 5 minutes each;

(5) Transparent: the slices are dehydrated with fresh absolute ethanol for 5 minutes, the xylene is transparent for 5 minutes, and the xylene is replaced with fresh xylene for 5 minutes.

(6) Mounting: Add neutral gum dropwise for mounting.

 Precautions

1. After staining, use a specific solution to remove the excessively bound dye solution from the tissue. This process is called differentiation, and the solution used is called differentiation solution. In HE staining species, low-concentration hydrocloric acid is often used as the differentiation solution, because the acid can destroy the quinoid structure of hematoxylin and separate the pigment from the tissue and fade. After the tissue is stained with hematoxylin, it must be differentiated to remove excessively bound and non-specifically adsorbed dyes in the tissue, before eosin staining can be performed to ensure that the nucleus and cytoplasm are clearly colored. G1039 hematoxylin differentiation solution can be used. During the differentiation process, the differentiation time should be appropriately adjusted according to the thickness of the tissue section, the type of tissue, etc. combined with observation under the microscope.

2. In HE staining, the bluishness after hematoxylin staining is very important. Hematoxylin is an ionic state under acidic conditions and is red; under alkaline conditions, it is blue. Tissue sections are red or pink after being differentiated by acidic differentiation solution. They need to be washed immediately to terminate differentiation, and then hematoxylin is made with weak alkaline solution. This process is the blue-returning effect. If there is no special blue-returning liquid, soaking with warm water can also make the nucleus blue, but it takes a little longer. Recommend G1040 hematoxylin blue liquid.

3. The dyeing degree of hematoxylin and eosin can be adjusted according to the dyeing needs.

4. Hematoxylin dye solution can be reused many times, and it should be sealed and stored after each use to prevent the active ingredients from volatilizing. When the tissues or cells are obviously lightly colored or the color is abnormal, please replace with a new dye solution.

5. The purity of the absolute ethanol used for dehydration after dyeing must be greater than 99.9%. If ethanol contains water, eosin will fade and cause uneven dyeing.

6. Eosin dye solution (alcohol-soluble) can be used repeatedly for several times. It is recommended to replace with a new dye solution when the tissue stain is obviously lighter.

7. Please wear lab coat and disposable gloves during operation.
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