Acid-Resistant Staining Solution for Microbial Morphology Staining Solution
China Acid-Resistant Staining Solution for Microbial Morphology Staining Solution, Find details about China Acid-Resistant Staining Solution, Morphology Staining Solution from Acid-Resistant Staining Solution for Microbial Morphology Staining Solution
Basic Info.
Model NO.
G1047-100ML
Habit Appellation
Special Reagent
Application
Industry, Scientific Research
Property
Biochemical Reagent
Trademark
Servicebio
Specification
100ML
Origin
China
Model NO.
G1047-100ML
Habit Appellation
Special Reagent
Application
Industry, Scientific Research
Property
Biochemical Reagent
Trademark
Servicebio
Specification
100ML
Origin
China
Product Desciption
Principle
Some acid-fast microorganisms such as Tubercle bacillus and Mycobacterium leprae is unable to be stained because of the lipoidal material in the cell wall. But by the application of carbol fuchsin, dye molecular penetrates through lipoidal wall into cytoplasm. This process makes all cell appearing red and it is not easy to discolor. Based on this characteristic, decolorize samples with decolorizing reagent and counterstain with hematoxylin followed by dehydrate. In this process, the acid fast cells are resistant to decolorize and retain the first staining color (red), while non-acid fast cells lack the lipoidal material in the cell wall due to which they are easily decolorized, leaving the cells colorless. Then the decolorized cells absorb the counterstain and appear blue.
Package content
Preservation
Valid in 3 months at room temperature.
Instructions
3,Rinse in 1% hydrocloric acid to differentiate for 1 s, wash in running tap water for 2 min. Observe the presence of positive purple-red microorganism under microscopy.
4,Differentiate in Sulfuric acid solution for 1 s, wash in tap water. Repeat the operations until positive microorganism show purple-red in colorless background.
5,Stain with hematoxylin solution for 1-3 min, differentiate in 1% hydrocloric acid, wash in tap water, then back to blue in amonia solution. Wash in running tap water.
Results
Acid-fast microorganisms are purple-red and cell nucleus are blue.
Notes
1,Strictly control the extent in the two steps of differentiate. Over-differentiate may result in decolorization of acid-fast microorganism, while inadequate differentiate results in red background.
2,Hematoxylin counterstain should be controlled lightly so as not to affect observing positive results.
Example
Tuberculosis bacillus are red while cell nucleus are blue.
Some acid-fast microorganisms such as Tubercle bacillus and Mycobacterium leprae is unable to be stained because of the lipoidal material in the cell wall. But by the application of carbol fuchsin, dye molecular penetrates through lipoidal wall into cytoplasm. This process makes all cell appearing red and it is not easy to discolor. Based on this characteristic, decolorize samples with decolorizing reagent and counterstain with hematoxylin followed by dehydrate. In this process, the acid fast cells are resistant to decolorize and retain the first staining color (red), while non-acid fast cells lack the lipoidal material in the cell wall due to which they are easily decolorized, leaving the cells colorless. Then the decolorized cells absorb the counterstain and appear blue.
Package content
| Code | Name | Size |
| G1047-1 | Carbol fuchsin | 10 ml |
| G1047-2 | Pheol solution | 90 ml |
| G1047-3 | Sulfuric acid solution | 100 ml |
| G1047-4 | Hematoxylin solution | 100 ml |
Valid in 3 months at room temperature.
Instructions
1,Deparaffinize and rehydrate to distilled water.
2,Immerse slides in Acid-fast staining solution for 30min, wash in running tap water.3,Rinse in 1% hydrocloric acid to differentiate for 1 s, wash in running tap water for 2 min. Observe the presence of positive purple-red microorganism under microscopy.
4,Differentiate in Sulfuric acid solution for 1 s, wash in tap water. Repeat the operations until positive microorganism show purple-red in colorless background.
5,Stain with hematoxylin solution for 1-3 min, differentiate in 1% hydrocloric acid, wash in tap water, then back to blue in amonia solution. Wash in running tap water.
- Dehydrate in 3 changes of absolute alcohol, 5 min each. Clear in 2 changes of xylene, 5 min each. Then mount with resin mounting medium.
Results
Acid-fast microorganisms are purple-red and cell nucleus are blue.
Notes
1,Strictly control the extent in the two steps of differentiate. Over-differentiate may result in decolorization of acid-fast microorganism, while inadequate differentiate results in red background.
2,Hematoxylin counterstain should be controlled lightly so as not to affect observing positive results.
Example
Tuberculosis bacillus are red while cell nucleus are blue.
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