Bone Tissue Safranin Green Staining Solution
China Bone Tissue Safranin Green Staining Solution, Find details about China Safranin Green Staining Solution, Bone Tissue Stain from Bone Tissue Safranin Green Staining Solution
Basic Info.
Model NO.
G1053-100ML
Habit Appellation
Special Reagent
Application
Industry, Scientific Research
Property
Biochemical Reagent
Trademark
Servicebio
Specification
100ML
Origin
China
Model NO.
G1053-100ML
Habit Appellation
Special Reagent
Application
Industry, Scientific Research
Property
Biochemical Reagent
Trademark
Servicebio
Specification
100ML
Origin
China
Product Desciption
Principle
Safranin O-Fast Green stain can well demonstrate cartilage layer and tidal line structures, excellently display structures of cartilago articularis, subchondral bone and bone tissues. This product stain cartilage matrix with homogenous red and green for bone with sharp contrast.
Package content
Preservation
Valid in 12 months at room temperature.
Procedure
1,Deparafiinize and rehydrate to distilled water.
2,Stain with Fast-Green solution 5-10 min, wash to remove the excess stain solution until cartilage is colorless. Rinse in hydrchloric acid alcohol slight to differentiate, wash in tap water.
5,Microscopy determination and analysis.
Results
Cartilage is red or orange in green background.
Notes
Safranin O-Fast Green stain can well demonstrate cartilage layer and tidal line structures, excellently display structures of cartilago articularis, subchondral bone and bone tissues. This product stain cartilage matrix with homogenous red and green for bone with sharp contrast.
Package content
| Code | Name | Size |
| G1053-1 | Safranin O solution | 100 ml |
| G1053-2 | Fast-Green solution | 100 ml |
Valid in 12 months at room temperature.
Procedure
1,Deparafiinize and rehydrate to distilled water.
2,Stain with Fast-Green solution 5-10 min, wash to remove the excess stain solution until cartilage is colorless. Rinse in hydrchloric acid alcohol slight to differentiate, wash in tap water.
- Stain in Safranin O solution for 13-30 s, dehydrate 3 changes in absolute alcohol.
5,Microscopy determination and analysis.
Results
Cartilage is red or orange in green background.
Notes
- If staining is unsuccessful, wash in water to clear fast-green and differentiate in hydrochoric acid alcohol to eliminate Safranin O. when all colors are cleared, restain slides starting from step 2.
- Staining time has a great impact on coloring degree. If the cartilages show significant green after stain in Fast-Green, differentiate in water is necessary to decolorize to light green or colorless. On the other way, if bone tissues show light color after differentiate, restain in Fast-Green and re-differentiate with water is necessary. The final aim is to show light green or colorless in cartilage while sharp green in bone tissues.
- Precipitations may occur in Safranin O when over-used, result in efficiency to stain. Please change fresh Safranin O solution in time.
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