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Ivd Lab Fungal Vaginal Microbial Pathogen Immunofluorescence Tissue Staining Solution

China Ivd Lab Fungal Vaginal Microbial Pathogen Immunofluorescence Tissue Staining Solution, Find details about China Gynecology Vaginal Microbe Staining Solution, Chitin Cellulose Glucan Mannose Fungus Staining from Ivd Lab Fungal Vaginal Microbial Pathogen Immunofluorescence Tissue Staining Solution

Model NO.
DH-ST01
Transport Package
Carton
Specification
25kit/box
Origin
China
HS Code
38220090
Model NO.
DH-ST01
Transport Package
Carton
Specification
25kit/box
Origin
China
HS Code
38220090
Ivd Lab Fungal Vaginal Microbial Pathogen Immunofluorescence Tissue Staining Solution
 

Clinic Signification

Vaginitis is a common disease in obstetrics and gynecology outpatient department, It mostly caused by various pathogens infection. Domestic epidemiological research shows that the most common is various type of vaginitis patients in gynecological outpatient department, accounting for more than 30% of the total. The Clinical common includes  bacterial vaginitis (22% - 50% of female symptoms), fungal vaginitis (17% - 39%), trichomonal vaginitis (4% - 35%), senile vaginitis and young female vaginitis.

It can significantly improve diagnosis experience and effect by standard and precise treatment , In clinic, It can screen different pathogen by testing the vaginal secretion .It can use different treatment methods by different pathogen infection, which is helpful for early diagnosis and treatment of vaginal diseases, It can reduce the incidence of complications and the risk of related diseases. It is of great social value to protect women's health and improve their fertility.
Ivd Lab Fungal Vaginal Microbial Pathogen Immunofluorescence Tissue Staining Solution
background and principle

 
The Immunofluorescence staining is a new staining solution for rapid detection of vaginal microbial pathogens. It is stained by the combination for the reagent fluorescence dye labelled antibody and the vaginal microbial pathogen , It is showed strong fluorescence under the excitation of ultraviolet light. It observe the fluorescence intensity and morphology w
 by fluorescence microscope ,so that it can judge the existence of vaginal microbial pathogens

By the principle of the immunology and fluorescence staining ,The reagent cassette can mark various cell , bacteria,fungal,and trichomonas in the vaginal secretion,  It can observe at the the same which use double beam UV and B under the fluorescence microscope in one glass slide ,it is used for the fluorescence immunomorphological examination for vaginal microbial pathogens

Application

   
This reagent is a IVD(in -vitro diagnostic) reagent  which used for the special staining treatment
of vaginal secretion samples, It is used for the staining of white blood cells, epithelial cells, clue cells, fungi, bacteria, trichomonas in vaginal secretions, which can be easy for observation and judgment.Ivd Lab Fungal Vaginal Microbial Pathogen Immunofluorescence Tissue Staining Solution


 Product Feature 
 
  1. It can clearly provide fungus, trichomonas, clue cell basis
  2.  It is more accurate than the humidity method for the mixed infection distinguish   It has more advantages in judging spores
  3. It is no effect for trichomonad inactivation so that can  reduce missed diagnosis
  4. It is more higher clinical coincidence rate than enzyme chemical method
  5. It is more simple and rapid than  gram staining method
  6. Comparison of diagnostic method of various pathogen
 Bacterial vaginitis colpomycosisTrichomonas vaginitis
Normal wet film normal saline
  •  
Because the bacterial morphology is small, the cell can not accurately observe the morphology of bacteria, so the high power microscope is not clear
  •  
The observation effect of fungal hyphae was ok, but the observation of spore was not satisfactory
  •  
it is easy to observe the Trichomonas flagellum movement state ,but when  inactivation or death ,its morphology is similar with leukocyte and  pus cells ,so that it is  difficult to distinguish
Gram staining methodAs for the clue cell observation ,Due to ordinary wet film,the sample was diluted,so that  the positive rate was reduced, and the operation was complicatedIt has the advantage of the contrast ratio for the observation of the fungal and germinal  spore , but the operation is complicated

 
  •  
  • The effect for observation trichomonas is normal, the operation is complicated
Rapid immunofluorescence staining method
  •  
  •  
Mark the clue cell using the fluorescein,one step staining ,fast time, strong fluorescence  contrast ratio,no need oil mirror,it can observe the complete bacterial form(including gardnerella, streotococcus,actinobacillus,long baccius ,etc),so that greatly improve the positive rate and accuracy ,it can meet the clinical test compliance rate
 		mark the fungal using the fluorescein one step staining ,fast time,strong contrast rate fluorescence ,big difference background,it is typical form for observing myceliaand germinal spore,low rate of missed diagnosis    mark the pathogen or cells using the fluorescein,one step staining ,fast time,strong fluorescence contrast rate ,big difference background, specific staining trichomonas ,it is not missed test duce to the inactivation or death  

Fungal is the large group of the eukaryotes, it has significantly different from plants , animals and bacteria.The gungal cell wall contain chitin ,cellulose ,glucan, mannose etc , the lower fungal cell wall is mainly composed of cellulose , yeast is mainly composed to the glucan ,the higher fungal is mainly composed to the chitin


 
The fungal infection is meaning that one or more pathogenic fungal invade the body tissues , it can cause the disease by the fungal or its metabolites .The range is from superficial infection (such as skin infection , mucosal ulcers) to deep tissue infection (such as severe lung infection ,sepsis , or systemic diseases )

 There are about more than 400kinds of pathogenic fungal , the main pathogenic fungal are classified as follows:
 
Superficial infection fungal surface infection fungal :angle layer tinea bacteria
Ringworm fungal:ringworm fungal,epidermophyton,small bun ringworm fungal
Deep infection fungal Subcutaneous tissue infection fungal:trichoderma spore,pigmentation fungal
Systemic fungal infection: Histoplasmosis, Coccidioidomycete, parasporidium, blastomycete
Opportunistic pathogenic fungal: Candida, Cryptococcus, Pneumocystis, aspergillus

Because of the lack of understanding of the biological characteristics of pathogenic fungal , In addtion, the variety of clinical manifestations of fungal infection, it is easy to miss diagnosis and misdiagnosis, cause the aggravation of the disease and even life-threatening, Therefore It need an effective and rapid early diagnosis method In clinic  

 Principle

The fluorescence brightener is one fluorescence dye,which has strong affinity with the chitin and cellulose of the fungal cell wall,It will emit bright blue fluorescence under the ultraviolet light (wavelength 340nm-380nm), it observe the morphology by the fluorescence microscope , so that it can fast detect the fungal

  Operation Progress Ivd Lab Fungal Vaginal Microbial Pathogen Immunofluorescence Tissue Staining SolutionIvd Lab Fungal Vaginal Microbial Pathogen Immunofluorescence Tissue Staining Solution


 Specification and clinical signification
  1. Simple quick-low technical requirement for the test person,it can read result after 2 minutes ,it can be easily decide in clinic
  2. High sensitivity --compare with the traditional direct microscopy method,high rate positive detection ,reduce the occurrence of false positive ,reduce the miss detection rate ,improve the accuracy of the test result
  1. High identification --it has a clear fluorescence under the fluorescent microscope , clear image outline , easy to observe, reduce the error of the operating person initiative judgement
  2. Wide application range :it can only detect certain fungal by the direct microscopy , but the fluorescence dye can be combination with all kinds of fungal ,then emit the fluorescence ,so it can all be detected of the shallow and deep infection fungal
Therefore , the fluorescence staining is a new rapid and effective method of testing fungal ,it not only provides the basis of rapid accurate fungal infection diseases,but also creates conditions for rapid diagnosis and rational drug use
l

 Interpretation of the result
Item Developing color  result judgement
1strong blue fluorescence ,identifiable morphology

 weak blue fluorescence,identifiable morphology 		--positive
2
  •  extremely weak fluorescence or no fluorescence
  •  
-negative

Technology comparison of common fungal diagnosis  
character
 test method 		immmunofluorescence KOHfungal incubationgram
Operational complexity  simple  simple  complication  complication
 test time  2-3minutes3-5 minutes2-3 days 1 hour
 positive detective rate  high low  low normal

 scope of application and specimen

 
The fungal immunofluorescence staining solution is mainly used for rapid tissue staining , it mainly stain for the fixation of formalin,paraffin-embedded tissue's fungal and the polysaccharides in the fungal cell wall
It is Suitable for samples of dander, nail polish, hair, sputum, body fluids and vaginal secretions

Collection method of common specimen 
  1. Skin specimen:firstly disinfect the diseased position with 75% alcohol ,select the moving edge of the lesion , and scrape the epidermal dander with a blunt scalpel
  2. Hair :detection of the brittle and loose hair from the root of the disease hair
  3. Sputum:take the morning sputum, if take the expectoration,it must brush your teeth on an empty stomach to disinfect your mouth  
  4. Vaginal secretions:use a long cotton sterilized swab, collect the white false membrane at the posterior vault of the vagina and cervix
  5. Ophthalmic specimen :as for the keratitis specimen , please use the ophthalmic spoon scrape the fester and ulcer specimen under the operation microscope , as for the conjunctiva specimen , it is dipped directly with the wet swab in the normal saline
 Fluorescence microscope
With infinite optical system ,UV LED is the main fluorescence exciation light source ,it can bring you a clear full field vision ,high contrast fluorescence image , meanwhile , Abbe concentrator increase the visibility and contrast of specimen , excellent image and simple operation ,it can widely used in the biological research , medical inspection and other filed,it can fast detect the fungal Ivd Lab Fungal Vaginal Microbial Pathogen Immunofluorescence Tissue Staining Solution
Specification and clinical signification
  1. Simple quick-low technical requirement for the test person,it can read result after 2 minutes ,it can be easily decide in clinic
  2. High sensitivity --compare with the traditional direct microscopy method,high rate positive detection ,reduce the occurrence of false positive ,reduce the miss detection rate ,improve the accuracy of the test result
  1. High identification --it has a clear fluorescence under the fluorescent microscope , clear image outline , easy to observe, reduce the error of the operating person initiative judgement
  2. Wide application range :it can only detect certain fungal by the direct microscopy , but the fluorescence dye can be combination with all kinds of fungal ,then emit the fluorescence ,so it can all be detected of the shallow and deep infection fungal
Therefore , the fluorescence staining is a new rapid and effective method of testing fungal ,it not only provides the basis of rapid accurate fungal infection diseases,but also creates conditions for rapid diagnosis and rational drug use
l

 Interpretation of the result
Item Developing color  result judgement
1strong blue fluorescence ,identifiable morphology

 weak blue fluorescence,identifiable morphology 		--positive
2
  •  extremely weak fluorescence or no fluorescence
  •  
-negative
 Fluorescence module
Ivd Lab Fungal Vaginal Microbial Pathogen Immunofluorescence Tissue Staining Solution


fluorescent module series products provides a new solution for laboratory fluorescence observation.With the LED fluorescence module, it is easy to convert a normal infinite optical  system microscope  into a modular, high efficiency  and easy-to-operate fluorescence microscope, enabling efficient fluorescence observation, do not need to purchase an expensive LED fluorescence microscope
the unique advantage
 
good developing effect  The background color is dark blue ,the fungal spores and mycelia are the bright blue , strong contrast ,developing effect better , reduce missed diagnosis
 
 high stability Unique stability processing technology, 100000 grade processing workshop to ensure the product stability , greatly reduce non-specific particles
 
 high sensitivity Strong bonding ability for the imported raw materials , short time reaction ,higher accuracy
 
Ivd Lab Fungal Vaginal Microbial Pathogen Immunofluorescence Tissue Staining SolutionIvd Lab Fungal Vaginal Microbial Pathogen Immunofluorescence Tissue Staining SolutionIvd Lab Fungal Vaginal Microbial Pathogen Immunofluorescence Tissue Staining Solution
 

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