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Medical Diagnostic Typhoid Antigen Test

China Medical Diagnostic Typhoid Antigen Test, Find details about China Typhoid Test from Medical Diagnostic Typhoid Antigen Test

Origin
China
Origin
China

Medical Diagnostic Typhoid Antigen Test

Intended Use
The One Step Typhoid IgG/IgM Test is a rapid chromatographic immunoassay for the qualitative detection of antibodies (IgG and IgM) to Typhoid virus in Whole Blood /Serum / Plasma to aid in the diagnosis of Typhoid viral infection.
Summary
Typhoid fever is a bacterial disease, caused by Salmonella typhi. It is transmitted through the ingestion of food or drink contaminated by the faces or urine of infected people. Symptoms usually develop 1-3 weeks after exposure, and may be mild or severe. They include high fever, malaise, headache, constipation or diarrhoea, rose-coloured spots on the chest, and enlarged spleen and liver. Healthy carrier state may follow acute illness. One step Typhoid IgG/IgM Test is a simple, visual qualitative test that detects Typhoid antibodies in human Whole Blood/serum/plasma. The test is based on immunochromatography and can give a result within 15 minutes.
Specimen Collection and Preparation
 
  1. The One Step Typhoid IgG/IgM Test can be performed using on Whole Blood / Serum / Plasma.
  2. To collect whole blood, serum or plasma specimens follow regular clinical laboratory procedures.
  3. For immediate near patient testing, please follow the finger prick testing procedure overleaf.
  4. Separate serum or plasma from blood as soon as possible to avoid haemolysis. Use only clear non-haemolysed specimens
  5. Testing should be performed immediately after specimen collection. Do not leave the specimens at room temperature for prolonged periods. Serum and plasma specimens may be stored at 2-8ºC for up to 3 days. For long term storage, specimens should be kept below -20ºC . Whole blood should be stored at 2-8ºC if the test is to be run within 2 days of collection. Do not freeze whole blood specimens.
  6. Bring specimens to room temperature prior to testing. Frozen specimens must be completely thawed and mixed well prior to testing. Specimens should not be frozen and thawed repeatedly.                                                                                                                                                                            Test Procedure
Allow the test, specimen, buffer and/or controls to reach room temperature 15-30ºC prior to testing.
  1. Wash hands with soap and rinse with warm water.
  2. Remove the test device from the sealed pouch and use it as soon as possible.
  3. Place the test device on a clean and level surface.
  4. Medical professionals should obtain a 35μl blood sample following their own clinical methods and proceed to point 9. Alternatively, take the safety lancet holding the body and twist off the protective cap until you feel it has been separated from the device. Don't pull, just twist.
  5. Clean the end of the forefinger or middle finger with an alcohol wipe. Massage the finger to enhance the blood flow.
  6. Press the safety lancet platform firmly against the chosen site.
  7. Keeping the hand down, massage the finger to obtain a blood drop.
  8. Without pressing the bulb, put the capillary tube in contact with the blood drop. The blood will migrate into the tube by capillary action. Fill to the black line indicated on the capillary tube trying to avoid getting any air bubbles in the tube.If necessary you may massage your finger again or use the second provided lancet to obtain more blood in order to fill the capillary tube.
  9. Transfer 1 drop of the collected blood (approximately 35μl) to the specimen well (S) of the test device, then add 2 drops of buffer (approximately 70μl) and start the timer. See illustration below:
  10. Wait for the coloured line(s) to appear. Read results at 15 minutes. Do not interpret the result after 20 minutes.
  11. Replace the protective cap and then dispose of lancet in a suitable container.
Notes
Applying sufficient amount of specimen is essential for a valid test result. If migration (the wetting of membrane) is not observed in the test window after one minute, add one more drop of buffer (for whole blood) or specimen (for serum or plasma) to the specimen well.
Interpretation of Results
Positive: Control line and at least one test line appear on the membrane. The appearance of T2 test line indicates the presence of Typhoid specific IgM antibodies. The appearance of T1 test line indicates the presence of Typhoid specific IgG antibodies. And if both T1 and T2 line appear, it indicates that the presence of both Typhoid specific IgG and IgM antibodies. The lower the antibody concentration is, the weaker the result line is.positive
Negative: One coloured line appears in the control region (C). No apparent coloured line appears in the test line region.
Invalid: Control line fails to appear. Insufficient specimen volume or incorrect procedural techniques are the most likely reasons for control line failure. Review the procedure and repeat the test with a new test device. If the problem persists, discontinue using the test kit immediately and contact your local distributor.


Medical Diagnostic Typhoid Antigen Test



Medical Diagnostic Typhoid Antigen Test






Medical Diagnostic Typhoid Antigen Test


 

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