Rnase Inhibitor for Molecular Biology Nucleic Acid Extraction
China Rnase Inhibitor for Molecular Biology Nucleic Acid Extraction, Find details about China Rnase Inhibitor, Enzyme Inhibitor from Rnase Inhibitor for Molecular Biology Nucleic Acid Extraction
Basic Info.
Model NO.
G3414-10KU
Habit Appellation
Chemical Medicine
Application
Industry, Scientific Research
Property
Biochemical Reagent
Trademark
Servicebio
Specification
10 KU
Origin
China
Model NO.
G3414-10KU
Habit Appellation
Chemical Medicine
Application
Industry, Scientific Research
Property
Biochemical Reagent
Trademark
Servicebio
Specification
10 KU
Origin
China
Product Desciption
Product Introduction
This product RNase Inhibitor (Ribonuclease Inhibitor), which is a ribonuclease inhibitor, is a pig-derived ribonuclease inhibitor recombinantly expressed by E. coli. It has a ratio of 1:1 with RNase A, B, and C in a non-competitive manner. The ratio is tightly combined to inhibit the activity of these RNases and protect RNA from degradation. This ribonuclease inhibitor has been proven to inhibit RNase in a variety of tissues and cells, but cannot inhibit the activities of RNase 1, H, T1, S1 Nuclease and RNase from Aspergillus. The performance of ribonuclease inhibitors from pigs and humans is very similar. The reaction system to maintain its inhibitory activity contains at least 1 mM DTT, the effective reaction pH is 5.0-8.0, and the optimal reaction pH is 7.0-8.0.
Uses: cDNA synthesis, in vitro RNA transcription, in vitro protein synthesis, etc.
Source: Ribonuclease from pigs, recombinantly expressed in E. coli.
Definition of enzyme activity: The amount of enzyme that can inhibit 50% of the activity of 5 ng RNase A is defined as one unit of enzyme activity.
Purity and concentration: SDS-PAGE detection purity ≥95%, 40 U/μL.
Inactivation or inhibition: completely inactivated at 75°C for 10 min. Protein denaturants such as SDS and Urea can inhibit the inhibition of RNase by RNase Inhibitor.
Enzyme storage buffer: 20 mM Tris-HCl, 50 mM NaCl, 5 mM DTT, 50% (v/v) glycerol, pH 7.5.
Storage and transportation
Transport with wet ice; storage at -20°C, valid for 12 months.
This product RNase Inhibitor (Ribonuclease Inhibitor), which is a ribonuclease inhibitor, is a pig-derived ribonuclease inhibitor recombinantly expressed by E. coli. It has a ratio of 1:1 with RNase A, B, and C in a non-competitive manner. The ratio is tightly combined to inhibit the activity of these RNases and protect RNA from degradation. This ribonuclease inhibitor has been proven to inhibit RNase in a variety of tissues and cells, but cannot inhibit the activities of RNase 1, H, T1, S1 Nuclease and RNase from Aspergillus. The performance of ribonuclease inhibitors from pigs and humans is very similar. The reaction system to maintain its inhibitory activity contains at least 1 mM DTT, the effective reaction pH is 5.0-8.0, and the optimal reaction pH is 7.0-8.0.
Uses: cDNA synthesis, in vitro RNA transcription, in vitro protein synthesis, etc.
Source: Ribonuclease from pigs, recombinantly expressed in E. coli.
Definition of enzyme activity: The amount of enzyme that can inhibit 50% of the activity of 5 ng RNase A is defined as one unit of enzyme activity.
Purity and concentration: SDS-PAGE detection purity ≥95%, 40 U/μL.
Inactivation or inhibition: completely inactivated at 75°C for 10 min. Protein denaturants such as SDS and Urea can inhibit the inhibition of RNase by RNase Inhibitor.
Enzyme storage buffer: 20 mM Tris-HCl, 50 mM NaCl, 5 mM DTT, 50% (v/v) glycerol, pH 7.5.
Storage and transportation
Transport with wet ice; storage at -20°C, valid for 12 months.
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