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Nucleic Acid Isolation & Purification Kit (MagPure FFPE RNA Kit)

China Nucleic Acid Isolation & Purification Kit (MagPure FFPE RNA Kit), Find details about China Rna/DNA Extraction Purification, Nucleic Acid Isolation from Nucleic Acid Isolation & Purification Kit (MagPure FFPE RNA Kit)

Model NO.
IVD3022
Content
Standard
Usage
Laboratory Reagents, Teaching Reagents
Source
Laboratory Produced
Habit Appellation
Chemical Reagent
Application
Research Use/for Lab
Property
Biochemical Reagent
Trademark
Magen Biotech
Specification
200 Preps/Kit
Origin
China
HS Code
3822009090
Model NO.
IVD3022
Content
Standard
Usage
Laboratory Reagents, Teaching Reagents
Source
Laboratory Produced
Habit Appellation
Chemical Reagent
Application
Research Use/for Lab
Property
Biochemical Reagent
Trademark
Magen Biotech
Specification
200 Preps/Kit
Origin
China
HS Code
3822009090
Product Description

Product Name Nucleic Acid Isolation & Purification Kit (MagPure FFPE RNA Kit)
Product specifications IVD3022,200Preps/Kit
Intended Use
This product is suitable for rapid extraction of RNA from tissue, cells, blood, s and other clinical samples.  RNA can be used directly for RT-PCR, quantitative RT-PCR, test of virus DNA and so on.

Principle
This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and Protease. DNA is released into the lysate. After adding magnetic particles and binding solution, DNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA was eluted by Elution Buffer.



Main Composition

Cat.No.IVD3022Composition
Purification times200 mlMagnetic Particles
MagPure Particles N7 ml 
DNase I4 x 600 µl 
DNase Buffer80 ml 
RTL Lysis Buffer150 ml 
Buffer MCB75 mlGuanidine Salt
Buffer MW188 ml 
Buffer MW250 mlGuanidine Salt
RNase Free Water60 mlTris/EDTA


Storage conditions and Validity
This product can be stored at room temperature (15~25ºC) for 12 months. MagPure RNA Particles, DNase I are shipped at room temperature. After received, store DNase I at -20ºC and store MagPure RNA Particles and proteinase K at 2~8ºC.



Materials and Equipment to be Supplied by User
1.Dilute Buffer MW1/MW2with absolute ethanol and store at room temperature
2.Dilute Buffer MCB store at room temperature
3.(Optional) Add 20µl 2-mercaptoethanol (or 2M DTT)per 1mL RTL Lysis Buffer. This mixture can be stored for 2 weeks at room temperature.




Auto Purify by KingFisher Flex or similar Extractor isolation:
1.Add the Reagents/sample to the well of f the deep well plate according to the table below.
Name of the PlatePre-loaded reagentsAddition before use
Sample plate500µl Buffer MCB
30µl MagPure RNA Particle		500µl cell lysate or lysate supernatant from step A.
Wash Plate 1600µl Buffer MW1, Put in 96 magnetic Tip
DNase290µl DNase Buffer and 10µl DNase I
Wash Plate 2600µl Buffer MW2
Wash Plate 3600µl Buffer MW2
Elution plate50~100µl RNase Free Water
2.Place a 96 tip comb for deep well magnets on Wash Plate 1.
3.Start the R6622_Flex protocol with the KingFisher Flex and load the plates.
4.Add 450µl Buffer MCB to the DNase plate during the dispense step.
5.Place the DNase plate back into the instrument and press Start. After the pause, the protocol will continue to the end.
6.After the run is completed, remove the plates and store the purified total RNA.
Packaging & Shipping

Nucleic Acid Isolation & Purification Kit (MagPure FFPE RNA Kit)Nucleic Acid Isolation & Purification Kit (MagPure FFPE RNA Kit)

Certificate

Nucleic Acid Isolation & Purification Kit (MagPure FFPE RNA Kit)

Laboratory

Nucleic Acid Isolation & Purification Kit (MagPure FFPE RNA Kit)

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Nucleic Acid Isolation & Purification Kit (MagPure FFPE RNA Kit)

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Nucleic Acid Isolation & Purification Kit (MagPure FFPE RNA Kit)

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