Magpure DNA Micro Kit

China Magpure DNA Micro Kit, Find details about China Rna/DNA Extraction Purification, Nucleic Acid Isolation from Magpure DNA Micro Kit

Basic Info.

Model NO.

IVD3101

Content

Standard

Usage

Laboratory Reagents, Teaching Reagents

Source

Laboratory Produced

Habit Appellation

Chemical Reagent

Application

Research Use/for Lab

Property

Biochemical Reagent

Trademark

Magen Biotech

Specification

200 Preps/Kit

Origin

China

HS Code

3822009090

Model NO.

IVD3101

Content

Standard

Usage

Laboratory Reagents, Teaching Reagents

Source

Laboratory Produced

Habit Appellation

Chemical Reagent

Application

Research Use/for Lab

Property

Biochemical Reagent

Trademark

Magen Biotech

Specification

200 Preps/Kit

Origin

China

HS Code

3822009090

Product Desciption

Product Name Nucleic Acid Isolation & Purification Kit

Product specifications IVD3101,200Preps/Kit
Intended Use
This product is suitable for rapid extraction of RNA from tissue, cells, blood, s and other clinical samples. RNA can be used directly for RT-PCR, quantitative RT-PCR, test of virus DNA and so on.

Principle
This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and Protease. DNA is released into the lysate. After adding magnetic particles and binding solution, DNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA was eluted by Elution Buffer.

Main Composition

Cat.No.	IVD3101	Composition
MagBind Particles	6.6 ml	Magnetic Particles
Proteinase K	90 mg	Protease
Protease Dissolve Buffer	10 ml	Glycorel/Tris/CaCl₂
Buffer ATL	60 ml	Tris/EDTA/SDS
Buffer AL	60 ml	Guanidine Salt
Buffer BD	20 ml	sodium perchlorate
Buffer BW1	110 ml	Guanidine Salt
Elution Buffer	30 ml	Tris/EDTA

Storage conditions and Validity
This kit is shipped and stored at room temperature and is valid for 12 months.

Materials and Equipment to be Supplied by User

1.Add 5ml ml Protease Dissolve Buffer to the Proteinase K, and store at -20~8ºC after dissolve

2.Add 2.5ml Protease Dissolve Buffer to the RNase A, and store at -20~8ºC after dissolve

3.Dilute Buffer GW1with 140ml 100% ethanol and store at room temperature

4.Dilute Buffer BD with 80ml 100% ethanol and store at room temperature

5.Prepare 75% Ethanol using Absolute Ethanol and store at room temperature.

Auto Purify by KingFisher Flex:

1.Add the Reagents/sample to the well of f the deep well plate according to the table below.

Name of the Plate	Pre-loaded reagents	Addition before use
Sample plate	400µl Buffer BD, 20µl MagBind Particle	400µl Lysis Sample or Supernatant
Wash Plate 1	500µl Buffer GW1, Put in 96 magnetic Tip
Wash Plate 2	500µl 75% ethanol
Wash Plate 3	500µl 75% ethanol
Elution plate	100µl Elution Buffer

2.Turn on the machine, start the corresponding program(IVD3101_F_96CE).

3.Place the 96-well plate into the instrument as prompted.
4.Finish the operation after ~30 minutes.
5.Remove the 96-well plate and magnetic jacket.
6.Store the Elute product at -20~8ºC.

Magpure DNA Micro Kit