Cat. No: P2091, 400 rxn/20μl reaction
P2092, 2000 rxn/20μl reaction

SYBR^® Green qPCR Mix
For research use only

Components  

Storage
This reagent can be stored for 2 months at 4°C and protected from light. For longer storage, it should be kept at -20°C and protected from light.

Description

SYBR Green qPCR 2X Mix is a premixed, ready-to-use solution containing Taq DNA Polymerase, dNTPs, MgCl₂, Reaction Buffer, Enzyme-linked antibody, SYBR Green I and other optimized buffer components at optimal concentrations for efficient amplification of DNA templates by quantitive real-time PCR. To prepare the final PCR, only primers and template DNA are added. SYBR Green qPCR Mix contributes to highly reproducible PCR by reducing the risk of pipetting errors, miscalculation and contamination. It also contributes to highly specific amplification by blocking the Taq DNA Polymerase at low temperature using the specific enzyme-linked antibody. The Mix is compatible with almost every brand of real-time fluorescence quantitative PCR instrument, such as Applied Biosystems, Eppendorf, Corbett, Bio-Rad and Roche.

Taq DNA Polymerase is purified from E. coli. expressing a cloned Thermus aquaticus DNA polymerase gene. This enzyme has a 5'→3' DNA polymerase and a 5'→3' exonuclease activity but lacks a 3'→5' exonuclease activity. Taq DNA polymerase is heat-stable and will synthesize DNA at elevated temperatures from single-stranded templates in the presence of a primer.

SYBR Green I (SG) is an asymmetrical cyanine dye used as a nucleic acid stain in molecular biology. SYBR Green I binds to double-stranded DNA. The resulting DNA-dye-complex absorbs blue light (λmax =497 nm) and emits green light (λmax=522 nm). SYBR green I is used as a dye for the quantification of double stranded DNA in some methods of real time PCR.

Applications

• Real-Time PCR

• Real-Time PCR RT-PCR

Features

• High specificity: Hot-start function and optimized buffer components to prevent non-specific amplification and the formation of primer dimers.

• Sensitivity: can amplify low copy templates.

• Wide linear range: accurately quantitative nine orders of magnitude.

• Compatibility: almost compatible with all real-time fluoresc-

ence quantitative PCR instrument.

• Convenient and reproducible: reducing the risk of pipetting errors, miscalculation and contamination.

Composition of the SYBR Green qPCR Mix 

100 mM KCl , 4 mM MgCl₂, 400 μM dNTPs, 0.1 U/μl Taq DNA Polymerase, 1x SYBR Green and other optimized buffer components