China Cell Culture Buffer Ca Mg Ebss Buffer with Phenol-Red, Find details about China Cell Culture Buffer, Ca Mg Ebss from Cell Culture Buffer Ca Mg Ebss Buffer with Phenol-Red
EBSS buffer, which is Earle's Balanced Salt Solution, is one of the commonly used phosphate buffers in cell separation or culture. The main components are glucose, NaHCO3, NaCl, KCl and Na2HPO4, which have the functions of maintaining osmotic pressure, maintaining pH stability, and providing simple nutrition. It can be used for rinsing tissue blocks, organs or cells, transporting cells or tissues, preparing reagents for cell culture and diluent for cell counting in vitro experiments. Calcium, magnesium ion and phenol-red indicator can be added as needed.
EBSS buffer is sterilized by 0.1 μm filter, pH 7.0-7.5@25ºC, osmotic pressure 270-310 mOsm/kg, containing calcium and magnesium ions, and phenol-red indicator. Please refer to the product formula table on the official website for the specific content of each ingredient.
EBSS(Earle's Balanced salt Solution)Formula | ||||||||
Cat.No. Components | G4213-500ML | G4214-500ML | G4215-500ML | G4216-500ML | ||||
mg/L | mM | mg/L | mM | mg/L | mM | mg/L | mM | |
CaCl2 (Water Free) | - | - | 200 | 1.80 | 200 | 1.80 | ||
MgSO4•7H2O | - | - | 200 | 0.81 | 200 | 0.81 | ||
KCl | 400 | 5.36 | 400 | 5.36 | 400 | 5.36 | 400 | 5.36 |
NaCl | 6800 | 117 | 6800 | 117 | 6800 | 117 | 6800 | 117 |
Water Free NaH2PO4 | 121.7 | 1.01 | 121.7 | 1.01 | 121.7 | 1.01 | 121.7 | 1.01 |
NaHCO3 | 2200 | 26.2 | 2200 | 26.2 | 2200 | 26.2 | 2200 | 26.2 |
D-glucose | 1000 | 5.55 | 1000 | 5.55 | 1000 | 5.55 | 1000 | 5.55 |
Phenol-Red | - | - | 10 | 0.025 | 10 | 0.025 |
Storage and Transportation
Transport at room temperature; store at 2-8°C, valid for 24 months.
Notes
1. The product can be used directly after 0.1 μm filtration.
2. Please pay attention to aseptic operation when using this product to avoid contamination.
3. Please wear lab coat and disposable gloves during operation