China Large Volume 500ml Hank′s Balanced Salt Solution, Find details about China Organ Rinse Buffer, Cell Count Diluent from Large Volume 500ml Hank′s Balanced Salt Solution
HBSS buffer, Hank's Balanced Solution, is one of the commonly used phosphate buffers in cell separation or culture. The main components are NaCl, KCl, KH2PO4, Na2HPO4, NaHCO3 and glucose, which have the functions of maintaining osmotic pressure, maintaining pH stability, and providing simple nutrition. It can be used for rinsing tissue blocks, organs or cells, transporting cells or tissues in vitro Preparation of reagents for cell culture and as a diluent for cell counting. Calcium, magnesium ion and chemical red indicator can be added as needed.
The product is sterilized by 0.1 μm filter, pH 7.2-7.5@25ºC, contains calcium and magnesium ions, and does not contain chemical red indicator.
Storage condition:
Stored at 4ºC, valid for 18 months.
Notes:
1. This product is sterilized by 0.1 μm filter and can be used directly.
2. Please pay attention to aseptic operation when using this product to avoid contamination.
3. This product contains calcium and magnesium ions, which are prone to micro-precipitation, which can be dissolved in a warm water bath. If there is a lot of precipitation that cannot be dissolved, please stop using it.
4. Hank's balanced salt solution cannot be used in a 5% CO2 culture environment. If placed in a CO2 incubator, the solution will quickly become acidic.
5. Please wear lab coat and disposable gloves during operation.