Description
Pfu 2X Mix Kit is a premixed, ready-to-use solution containing Pfu DNA Polymerase, dNTPs and Reaction Buffer at optimal concentrations for efficient amplification of DNA templates by PCR. In this kit, Pfu DNA Polymerase is stored separately from PCR reaction Mix. To prepare the final PCR, please mix the polymerase and the reaction Buffer in appropriate proportion, and then add the primers and template DNA needed. Pfu Mix Kit contributes to highly reproducible PCR by reducing the risk of pipetting errors, miscalculation and contamination. It also contributes to higher sensitivity by adding enhancer. Using the kit in your PCR reaction results in blunt-ended PCR products.

Pfu DNA polymerase, derived from the hyperthermophilic archae Pyrococcus furiosus, has been shown to exhibit superior thermostability and proofreading properties compared to other thermostable polymerase.  Unlike Taq DNA polymerase, highly thermostable Pfu DNA polymerase possesses 3' to 5' exonuclease proofreading activity that enables the polymerase to correct nucleotide-misincorporation errors. This means that Pfu DNA polymerase-generated PCR fragments will have fewer errors than Taq-generated PCR inserts. Using Pfu DNA polymerase in your PCR reactions results in blunt-ended PCR products, which are ideal for cloning into blunt-ended vectors. Pfu DNA polymerase is superior for techniques that require high-fidelity DNA synthesis.

Features
Convenient: only primers and template are needed
Efficiency: simplifying the operation and saving your time
Reproducible: reducing the risk of pipetting errors, miscalculation and contamination
Flexible  : the amount of polymerase is flexible and controllable

Applications
High throughput, high reproducible routine PCR
High fidelity PCR

Unit Definition
One unit is defined as the amount of the enzyme required to catalyze the incorporation of 10 nmoles of dNTP's into an acid-insoluble form in 30 minutes at 70°C using hering sperm DNA as substrate.

2×PCR Reaction Mix
100 mM KCl, 200 mM Tris-Cl, 20 mM MgSO₂, 100mM(NH₄)₂SO₄,400 μM dNTPs, bromophenol blue

Storage buffer
20 mM Tris-HCl, 1 mM DTT, 0.1 mM EDTA, 100 mM KCl, 0.5 % TW 20, 0.5 % NP 40, 50 % Glycerol

Store all components at -20°C