Basic Info.

Model NO.

P1021

Model NO.

P1021

Product Desciption

Pfu DNA Polymerase
#P1021, 250U

 

Concentration: 2.5U/μl
Contents:

Pfu DNA Polymerase	100 μl
10× Pfu Buffer (Mg2+ Free)	1.25 ml
6× Loading Buffer	1 ml
25mM MgCl2	1.25 ml

Store at -20°C
For research use only.

Description
Pfu DNA polymerase, derived from the hyperthermophilic archae Pyrococcus furiosus, has superior thermostability and proofreading properties compared to other thermostable polymerase. Its molecular weight is 90 kDa. It can amplify DNA target up to 2 kb(simple template). The elongation velocity is 1kb/min(70~75°C). Pfu DNA polymerase possesses 3' to 5' exonuclease proofreading activity that enables the polymerase to correct nucleotide-misincorporation errors. This means that Pfu DNA polymerase-generated PCR fragments will have fewer errors than Taq-generated PCR inserts. Using Pfu DNA polymerase in your PCR reactions results in blunt-ended PCR products, which are ideal for cloning into blunt-ended vectors. Pfu DNA polymerase is superior for techniques that require high-fidelity DNA synthesis.
Unit Definition
One unit is defined as the amount of the enzyme required to catalyze the incorporation of 10 nmole of dNTPs into an acid-insoluble form in 30 minutes at 70°C using hering sperm DNA as substrate.
Storage Buffer
20mM Tris-HCl (pH 8.0), 100mM KCl, 3mM MgCl_2, 1mM DTT, 0.1% NP-40, 0.1% Tween20, 0.2mg/ml BSA, 50% (V/V) glycerol.
Applications
• High-fidelity PCR and primer-extension reactions
• High fidelity PCR for cloning into blunt-ended vectors
• Site-directed mutagenesis

Quality Control
The absence of endodeoxyribonucleases, exodeoxyribonucleases and ribonucleases is confirmed by appropriate quality tests. Functionally tested in amplification of a single-copy gene from human genomic DNA.
Endodeoxyribonuclease Assay
No detectable conversion of covalently closed circular DNA to a nicked DNA was observed after incubation of 10U Pfu DNA Polymerase with 1μg pBR322 DNA for 4 hours at 37°C  and 70°C.
Exodeoxyribonuclease Assay
No detectable degradation of lambda DNA-HindIII fragments was observed after incubation of 10U Pfu DNA Polymerase with 1μg digested DNA for 4 hours at 37°C and 70°C.
Ribonuclease Assay
0% of the total radioactivity was released into trichloroacetic acid-soluble fraction after incubation of 10U Pfu DNA Polymerase with 1μg E.coli [3H]-RNA (40000cpm/μg) for 4 hours at 37°C and 70°C.





Dongsheng Biotech offers different series of products to help you achieve PCR success. For enzymes, our Taq Polymerase, HS Taq DNA Polymerase, FS Taq DNA Polymerase, Pfu DNA Polymerase and Fusion Pfu DNA Polymerase provide high fidelity, efficient, sensitivity PCR performance. We also have Long Taq DNA Polymerase for long PCR performance.