Von Kossa Stain Reagent for Calcium Nodules in Animal Tissues
China Von Kossa Stain Reagent for Calcium Nodules in Animal Tissues, Find details about China Von Kossa Stain, Calcium Nodules Stain from Von Kossa Stain Reagent for Calcium Nodules in Animal Tissues
Basic Info.
Model NO.
G1043-20ML
Habit Appellation
Special Reagent
Application
Industry, Scientific Research
Property
Biochemical Reagent
Trademark
Servicebio
Specification
3× 20 mL
Origin
China
Model NO.
G1043-20ML
Habit Appellation
Special Reagent
Application
Industry, Scientific Research
Property
Biochemical Reagent
Trademark
Servicebio
Specification
3× 20 mL
Origin
China
Product Desciption
Principle
Von Kossa staining is used to detect calcium deposition in tissues based on a replacement reaction. The major component is silver nitate. In this method, sections are treated with silver solution and the silver is deposited by replacing the calcium reduced by strong light, resulted in visualized black depositions.
Package content
Preservation
Valid in 6 months at room temperature. In addition, Von Kossa should be kept in dark place.
Instructions
1,Deparaffinize paraffin sections and hydrate to water. Rinse in 3 changes of distilled water.
2,Silver nitate stain. Add silver nitate solution to cover sections. Place sections under ultraviolet light for 2-3 h. Rinse in several changes of distilled water.
3,Hematoxylin stain. Immerse sections in hematoxylin solution for 3-5 min, differentiate with 1% hydrocloric acid, back to blue in amonia solution, then wash in tap water.
4,Dehydrate in 85%, 95% alcohol respectively, 5 min each. Counterstain with eosin solution for 5 min.
5,Dehydrate in 3 changes of absolute alcohol, clear in 2 changes of xylene and mount with resin mounting medium.
6,Microscopy determination and image acquisition for analysis. Results
The calcium deposition is black or brown black in red background.
Notes
1,Slides should be washed clearly before silver nitate staining.
2,Strong sunlight is alternative if ultraviolet is unavailable.
Example
Von Kossa staining is used to detect calcium deposition in tissues based on a replacement reaction. The major component is silver nitate. In this method, sections are treated with silver solution and the silver is deposited by replacing the calcium reduced by strong light, resulted in visualized black depositions.
Package content
| Code | Name | Size |
| G1043-1 | Von Kossa solution | 20 ml |
| G1043-2 | Hematoxylin solution | 20 ml |
| G1043-3 | Eosin solution | 20 ml |
Valid in 6 months at room temperature. In addition, Von Kossa should be kept in dark place.
Instructions
1,Deparaffinize paraffin sections and hydrate to water. Rinse in 3 changes of distilled water.
2,Silver nitate stain. Add silver nitate solution to cover sections. Place sections under ultraviolet light for 2-3 h. Rinse in several changes of distilled water.
3,Hematoxylin stain. Immerse sections in hematoxylin solution for 3-5 min, differentiate with 1% hydrocloric acid, back to blue in amonia solution, then wash in tap water.
4,Dehydrate in 85%, 95% alcohol respectively, 5 min each. Counterstain with eosin solution for 5 min.
5,Dehydrate in 3 changes of absolute alcohol, clear in 2 changes of xylene and mount with resin mounting medium.
6,Microscopy determination and image acquisition for analysis. Results
The calcium deposition is black or brown black in red background.
Notes
1,Slides should be washed clearly before silver nitate staining.
2,Strong sunlight is alternative if ultraviolet is unavailable.
Example
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