Delta Nucleic Acid Fluorescence Real Time PCR Test Reagent Kit

China Delta Nucleic Acid Fluorescence Real Time PCR Test Reagent Kit, Find details about China Delta PCR Test Reagent Kit, Delta PCR Test Reagent from Delta Nucleic Acid Fluorescence Real Time PCR Test Reagent Kit

Basic Info.

Model NO.

R039-03

HS Code

3822009020

Model NO.

R039-03

HS Code

3822009020

Product Desciption

For mutant virus Delta, we use the magnetic bead method, and higher sensitivity can guarantee no missed detection. Our high-precision super-paramagnetic nanometer magnetic bead method has a large surface area of the magnetic bead, and the magnetic bead is attached to the nucleic acid affinity group, and the adsorption efficiency is high. Only one wash or direct amplification with magnetic beads is required, which greatly reduces the loss of nucleic acid and ensures the accuracy, rapidness, simplicity, and high throughput of detection reagents, which can meet the needs of large-scale nucleic acid screening and avoid missed detection.

Product Description
The kit is specially used for in vitro qualitative detecting the ORF1ab/N
genes in respiratory specimens including oropharyngeal swabs, nasopharyngeal swab, sputum and bronchoalveolar lavage fluid, and performing D950N mutation detection of Delta variant.
As reported in GISAID database, more than 95% Delta variant carry D950N mutation. Primer sets and FAM labeled probe are designed for specific detection of ORFlab gene, VIC labeled probe for N
gene, ROX labeled probe for D950N mutation of Delta variant. Human RNase P gene extracted concurrently with the test sample provides an internal control to validate nucleic extraction procedure and reagent integrity. Probe targeting human RNase P gene is labeled with CY5.

Size
50 tests/kit

Kit Contents
RT-PCR reaction buffer 925 μL × 1 tube
RT-PCR enzyme mix 75μ L × 1 tube
Positive control 100 μL × 1 tube
Negative control 100 μL × 1 tube
Precautions:
1. Components with different lot numbers cannot be used together.
2. User prepared RNA extraction Kit.

Storage and Expiration
This kit expires in 12 months when stored at -20+-5 Celsius and in
prevention of light.
Avoid repeated freeze-thaw cycles (5 times maximum).Manufacture date and expiration date are printed on the label.

Applicable Instruments
Real-Time PCR System: Molarray MA-6000, ABI 7500, ViiATM 7,
QuantStudio 5, QuantStudio 6/7 pro, QuantStudio 6/7 flex, Agilent
Mx3000P/3005P, Rotor-GeneTM 6000/Q, Bio-Rad CFX96
TouchTM/iQTM 5, Hongshi SLAN-96S/96P, Daan AGS8830,
AGS4800

Specimen Handling and Storage
1. Acceptable Specimens: oropharyngeal swab, sputum,
bronchoalveolar lavage fluid and nasopharyngeal swab.
2. Collect samples in sterile tubes.
3. Contamination should be avoided during collection, storage and
transportation of the samples.
4. All the samples collected should be treated as contagious,
deactivate the sample at 56 Celsius for 30 min.
5. Specimens can be stored at 2-8 Celsius for up to 24 hours after
collection. If a delay in extraction is expected, store specimens at
-70 Celsius or lower. Avoid repeated freeze-thaw cycle of the sample and make sure sample is completely thawed before RNA extraction.
6. Transport samples in sealed box with dry ice or ice bag.

Description of RT-PCR reagent
Name of product	RT-PCR reagent(ORF1ab, N,D950N)
Specification	(ORF1ab, N)/(ORF1ab, N,D950N)
Package	50 tests/kit, (5,000tests+16ice bags)/ctn
Carton size(cm)	534346
CBM	0.105
N.W.	3.0 kg
G.W.	16.0 kg
Loading port	Shenzhen/Guangzhou/Hongkong
MOQ	10,000 tests
FOB price	negociate