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Goldner Three-Color Method Staining Solution for Nucleus Collagen Fiber

China Goldner Three-Color Method Staining Solution for Nucleus Collagen Fiber, Find details about China Goldner Staining, Cell Nucleus Staining from Goldner Three-Color Method Staining Solution for Nucleus Collagen Fiber

Model NO.
G1064-20ML
Application
Industry, Scientific Research
Property
Biochemical Reagent
Trademark
Servicebio
Specification
20ML
Origin
China
Model NO.
G1064-20ML
Application
Industry, Scientific Research
Property
Biochemical Reagent
Trademark
Servicebio
Specification
20ML
Origin
China
Goldner Three-Color Method Staining Solution for Nucleus Collagen FiberGoldner Three-Color Method Staining Solution for Nucleus Collagen FiberPrinciple
Goldner trichrome staining is mainly used to differentiate mineralized and non-mineralized bone based on dye molecular weight and tissue permeability. The conformation of collagen fibers changed in mineralized bone which can be stained by dye. As a result of this method, the mineralized bone is green while non-mineralized bone is orange, other elements such as muscles, cytoplasm and red blood cells are red, nucleus are purple-black or grey-black. It is applied in evaluate the porosis in bone defect repairing.
Package content
Code Name Size
G1064-1Hematoxylin iron  solution A20ml
G1064-2Hematoxylin iron solution 20ml
G1064-3Acid ponceau fuchsin solution20ml*2
G1064-4Organge G solution20ml
G1064-5Brilliant green solution20ml
Preservation
Valid in 6 months at room temperature
Procedure
1 Deparaffinize and rehydrate by immersing the slides through the following:
  1. Xylene: two washes 20 minutes each
  2. 100% alcohol: two washes for 5 minutes
  3. 75% alcohol: wash for 5 minutes
  4. Wash briefly in tap water
  1. Iron hematoxylin stain Mix up the Weigert hematoxylin iron solution A with solution B by equivalent volume to generate iron hematoxylin solution. Rinse the slides in this iron hematoxylin solution for 20 minutes, wash in tap water. Then differentiate in 1% acid alcohol less than 5 seconds, wash in distilled water.
  2. Acid ponceau fuchsin stain Stain in acid ponceau fuchsin solution for 5-10 minutes, rinse in glacial acetic acid (0.1-1%) to wash quickly. 
4 Orange G stain. Rinse in Organge G solution for 1-5 min. manage the time by observing in microscopy until the ponceau of collagen fade.
5 Brilliant green stain. Rinse slide in Brilliant green solution for 3-10 min, differentiate in three changes of glacial acetic acid (0.1-1%). Then dehydrate in 3 changes of absolute alcohol, 5 min each. 
6 Clear in xylene for 5 min and mount with resin mounting medium. 
7 Microscopy examination and analysis

Results
Collagen fibers are blue. Muscle fibers, cellulose and red blood cells are red.

Notes
1,Change the iron hematoxylin solution in time according to slides amouns and usage. Prepare before use is alternative.  
2,control the extent of staining with Orange G and acid ponceau fuchsin. Too deep red of collagen has negative impact on Brilliant green coloring effect.
3,control the proper differentiate extent in glacial acetic acid. Avoid inadequate or over differentiate.
Goldner Three-Color Method Staining Solution for Nucleus Collagen FiberGoldner Three-Color Method Staining Solution for Nucleus Collagen FiberGoldner Three-Color Method Staining Solution for Nucleus Collagen FiberGoldner Three-Color Method Staining Solution for Nucleus Collagen FiberGoldner Three-Color Method Staining Solution for Nucleus Collagen Fiber

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