CE Proved Hipure Circulating DNA Kit Free-Circulating Nucleic Acid Isolation & Purification Kit

China CE Proved Hipure Circulating DNA Kit Free-Circulating Nucleic Acid Isolation & Purification Kit, Find details about China Sample Tube, Circulating DNA Extraction from CE Proved Hipure Circulating DNA Kit Free-Circulating Nucleic Acid Isolation & Purification Kit

Basic Info.

Model NO.

IVD3182

Content

Standard

Usage

Laboratory Reagents, Teaching Reagents

Source

Laboratory Produced

Habit Appellation

Chemical Reagent

Application

Research Use/for Lab

Property

Biochemical Reagent

Trademark

Magen Biotech

Specification

50 Preps/Kit

Origin

China

HS Code

3822009090

Model NO.

IVD3182

Content

Standard

Usage

Laboratory Reagents, Teaching Reagents

Source

Laboratory Produced

Habit Appellation

Chemical Reagent

Application

Research Use/for Lab

Property

Biochemical Reagent

Trademark

Magen Biotech

Specification

50 Preps/Kit

Origin

China

HS Code

3822009090

Product Desciption

Magen's Nucleic Acid Isolation &Purification Kits Germany in-Vitro Diagnostic registered (98/79/EC) Chinese in vitro diagnostic Reagent Glass I, 20150062)
Function Name	Cat.No.	Quality (Preps)	Price	Nucleic Acid Type	Method	Sample	Applicaton
HiPure Universal DNA Kit	IVD3018	100		genomic DNA	Silica-gel Column	10~200ul Blood, Plasma, Saliva, Body fluids 1~20mg Tissue, <3 x 10⁶ Culture Cells Dry Blood Spot and swab FFPE sample	PCR, Real time PCR NGS Library prepare
HiPure Circulating DNA Kit	IVD3182	50		Circulating DNA		1~5 ml Plasma, Serum, cerebral effusion, ascites, cell culture supernatants.	PCR, Real time PCR NGS Library prepare
HiPure Viral DNA/RNA Kit	IVD4173	100		Viral Nucleic Acid		~200ul Plasma, Serum, Swab Soak, Tissue homogenate superatant, other body fluids	Real Time PCR PCR
HiPure Viral RNA Kit	IVD4175	100		Viral RNA		~200ul Plasma, Serum, Swab Soak, Tissue homogenate superatant , other body fluids	Real time PCR PCR
MagPure Universal DNA Kit	IVD3102	200		genomic DNA	Magnetic Particles	10~200ul Blood, Plasma, Saliva, Body fluids 1~20mg Tissue, <3 x 10⁶ Culture Cells Dry Blood Spot and swab FFPE sample	PCR, Real time PCR NGS Library prepare
MagPure Viral DNA/RNA Kit	IVD5412 (Bottle package)	200		Viral Nucleic Acid		~200ul Plasma, Serum, Swab Soak, Tissue homogenate superatant, other body fluids	Real Time PCR PCR
	IVD5412-F-96 (Precast for kingFisher Flex)	96
	IVD5412-TL-96 (Precast for 32 channel Machine)	96
MagPure Stool DNA Kit	D6364-400	400		Stool DNA		Isolation Total DNA include microbial DNA from ~150mg Stool Samples.	PCR, Real time PCR NGS Library prepare

Product Name Nucleic Acid Isolation & Purifiation Kit(HiPure Circulating DNA Kit )
Product specifications IVD3182,50 Preps/Kit
Intended Use
Free-circulating nucleic acids, such as tumor-specific extracellular DNA fragments and mRNAs in the blood or fetal nucleic acids in maternal blood, are present in serum or plasma usually as short fragments, <1000bp(DNA). HiPure Circulating DNA Midi Kit enables efficient purification of these circulating nucleic acids from human plasma, serum, or urine.The extracted products can be used for clinical in vitro detection.
Principle
This product is based on silica gel purification. The sample is lysed and digested with lysate and protease, DNA is released into the lysate. Transfer to an adsorption plate and filter column. Nucleic acid is adsorbed on the membrane, while protein is not adsorbed and is removed with filtration. After washing proteins and other impurities, Nucleic acid was finally eluted with low-salt buffer (10 Mm Tris, pH 8.0).

Main Composition

Cat.No	IVD3182	Contents
Buffer ACL	250 ml	Glycerol/Tris/CaCl2
Buffer ACB*	300 ml	Tris/EDTA/Guanidine Salt
Buffer DCW1*	22 ml	Tris/EDTA/Guanidine Salt
Buffer DCW2*	10 ml	Tris/Nacl
Proteinase K	540 mg	10mm Tris,pH8.0
Protease Dissolve Buffer	30 ml	Glycerol/Tris/CaCl2
Carrier RNA	110 µg	Poly A
Nuclease Free Water	20 ml	Water
HiPure CFDNA Mini Columns	50	Silicon Column
2 ml Collection Tubes	100	PP Column
Extender Tube	50	PP Column
Vac-Connector	50	PS Column

Storage conditions and Validity
This kit is shipped and stored at room temperature and is valid for 12 months.

Reagent volume follow the table

Sample volumes	1ml	2ml	3ml	4ml	5ml
Proteinase K	100µl	200µl	300µl	400µl	500µl
Buffer ACL	0.8ml	1.6ml	2.4ml	3.2ml	4.0ml
Carrier RNA	5µl	5µl	5µl	5µl	5µl
Buffer ACB	1.8ml	3.6ml	5.4ml	7.2ml	9ml
Buffer DCW1	750ul	750ul	750ul	750ul	750ul
Buffer DCW2	750ul	750ul	750ul	750ul	750ul
100% Ethanol	750ul	750ul	750ul	750ul	750ul

Troubleshooting Guide
1. Low or no recovery

Buffer DCW1/DCW2/ACB did not contain ethanol/Isopropanol: Ethanol/Isopropanol must be added to Buffer DCW1/DCW2/ACB before used. Repeat procedure with correctly prepare Buffer.
Low concentration of target DNA in the Sample:Samples were standing at room temperature for too long. Repeated freezing and thawing should be avoided. Anticoagulants other than EDTA may lead to accelerated DNA degradation.

2. DNA does not perform well (e.g. in ligation reaction)

Salt concentration in eluate too high: Modify the wash step by incubating the column for 5 min at room temperature after adding 650ul of Buffer DCW2, then centriufge or Vacuum.
Eluate contains residual ethanol: Ensure that the wash flow-through is drained from the collection tube and that the column is then centrifuged at >12,000 x g for 1min, then dry.

Inappropriate elution volume used: Determine the maximum volume of eluate suitable for your amplification reaction. Reduce or increase the volume of eluate added to the amplification reaction accordingly.

3. Clogged HiPure cfDNA Mini Column

Vacuum pressure of 800-900mbar not reached: The vacuum manifold is not tightly closed.
Transfer the remaining sample lysate to a new tube, place the column in a new collection tube and spin it at full speed for 1 min.