China Nucleic Acid Isolation & Purification Kit with Silica Gel Purification, Find details about China Rna/DNA Extraction Purification, Nucleic Acid Isolation from Nucleic Acid Isolation & Purification Kit with Silica Gel Purification
Product Name Nucleic Acid Isolation & Purification Kit
Product specifications IVD4121,50Preps/Kit
Intended Use
This kit is used for extracting total RNA from non-cell/low cell content biological samples such as body fluid, serum, plasma, immersion solution, tissue homogenate supernatant, culture supernatant, etc., the extracted products can be used for clinical in vitro detection.
Principle
This product is based on silica gel purification. The sample is lysed and digested with lysate and protease, DNA / RNA is released into the lysate. Transfer to an adsorption plate and filter column. DNA/RNA is adsorbed on the membrane, while protein is not adsorbed and is removed with filtration. After washing proteins and other impurities, DNA / RNA was finally eluted with low-salt buffer (10 Mm Tris, pH 8.0).
Main Composition
Cat.No. | IVD4121 | |
HiPure DNA Mini Column | 50 | HiPure DNA Mini Column |
HiPure RNA Mini Columns | 50 | HiPure RNA Mini Columns |
2ml Collection Tubes | 100 | 2ml Collection Tubes |
Proteinase K | 24 mg | Protease |
Protease Dissolve Buffer | 1.8 ml | Glycorel/Tris/CaCl2 |
DNase I | 600 µl | DNase I |
DNase Buffer | 6 ml | Tris/CaCl2 |
Buffer RTL | 30 ml | Guanidine Salt |
RNA Digestion Buffer | 15 ml | Guanidine Salt |
Buffer RWC* | 20 ml | Guanidine Salt |
Buffer RW2* | 25 ml | Tris/NaCl |
RNase Free Water | 10 ml | DEPCWater |