Product Introduction

DMEM/High Glucose, or DMEM (Dulbecco's Modified Eagle Medium) high glucose medium, is an improvement on the basis of MEM medium and has been widely used in the cultivation of various cells. DMEM high-sugar medium is generally used for cells that grow fast and have low adhesion. It can be used for the cultivation of a variety of mammalian primary cells, such as primary fibroblasts, nerve cells, glial cells, HUVEC, smooth muscle cells, etc., and can also be used for the cultivation of some cell lines, such as Hela, 293, Cos- 7 and PC-12 etc.

This product contains amino acids, vitamins, inorganic salts and other ingredients required for cell culture. It does not contain protein or growth factors. It is necessary to add 5-10% serum or serum-free additives according to the cell type.

This product is sterilized by filtration through a 0.1 μm filter membrane, pH 7.0-7.4, containing 25 mM glucose, adding 4.0 mM GlutaMAX-I, containing phnol red indicator, not containing sodium BT, and containing 25 mM HEPES buffer system.

GlutaMAX-I, or L-alanyl-L-glutamine, is a cell culture additive that can replace L-glutamine in cell culture media. The substance is very stable in aqueous solution and will not degrade spontaneously. Instead, it is slowly degraded by a peptidase secreted by cells to release glutamine, which is then absorbed and utilized by cells.

HEPES is an excellent biological buffer, which has no toxic effect on cells. The medium added with HEPES can maintain a constant pH range for a long time.


Storage and transportation

Transported in wet ice, stored at 2-8°C, valid for 12 months.


Precautions

1. This product has been filtered and sterilized. When using it, you should pay attention to aseptic operation to avoid contamination;

2. In order to maintain the best results, please do not freeze-thaw.