Medical YSPCR-Q96 Real-time Quantitative PCR
YSPCR-Q96 real-time quantitative PCR system is based on global vision of product design concepts and manufacturing processes. It creatively combines Fresnel lens optical signal acquisition technology, time-resolved signal separation technology and unique temperature control technology. And it reaches inte DNAtional advanced level in sensitivity, multi-color crosstalk, temperature uniformity and accuracy. It supports the application of all common QPCR detection modes.
Up to 6 fluorescence detection channels meet the requirement of multiplex PCR
Effectively reduce multi-color crosstalk and edge effect
New optical scanning detection system--High sensitivity /resolution
Innovative scanning method and time-resolved signal separation technology--High accuracy
Unique edge temperature compensation technology
Unique design, user-friendly operating software
One-stop process and solution
Classic examplesProgram:Step | Temperature | Time | Cycle | End point |
1 | 42ºC | 20min | 1 | No |
2 | 95ºC | 10min | 1 | No |
3 | 94ºC | 15sec | 40 | No |
55ºC | 30sec | Yes |
Judgment basis:
Positive: Ct≤35,and the amplification curve has a significant exponential growth curve.
Negative:Ct>37,or no significant amplification of the curve.Recommended retest: 35
Amplification curve:
Technical ParametersTemperature control system |
Sample capacity | 0.2ml PCR tubes×96, 8×12 PCR plate or 96 well plate ×1 |
Reaction volume | 10-50 μl |
Thermal cycle technology | Peltier |
Max. Heating/Cooling rate | 6.0° C/s |
Heating temperature range | 4 - 100 °C |
Temperature accuracy | ± 0.2°C |
Temperature uniformity | ±0.2ºC @60ºC , ±0.3ºC @95ºC |
Temperature gradient setting range | 30-100°C |
Temperature gradient difference setting range | 1 - 36 °C |
Detection system |
Excitation light source | 4/6 monochrome high efficiency LEDs |
Detection device | PMT |
Detection mode | Time-resolved signal separating technology |
Excitation/detection wavelength range | 455-650nm/510-715nm |
Fluorescent channels | 4/6 channels |
Supported dye | FAM/SYBR Green, VIC/JOE/HEX/TET, ABY/NED/TAMRA/Cy3, JUN, ROX/Texas Red, Mustang Purple, Cy5/LIZ |
Sensitivity | Single copy gene |
Resolution | 1.33 folds copy number difference can be distinguished in single-plex qPCR |
Dynamic range | 10 orders of magnitude copies |
Related products:Portable PCR gradient thermocycler thermal cycler Features:
• Widely used in molecular biology, clinical diagnosis, criminal investigations and infectious disease research etc.
• Provides consistent and uniform sealing across an entire block
• Excellent temperature control system
• Fast heating and cooling rate
• Intuitive touch-screen user interfaceSpecificationsSample Capacity | 0.2ml PCR tubes×96, 1PCR plate 8×12 or 96 well plate x1 |
Heating Temperature Range[oC] | 4-99 |
Lid Temperature Range[oC] | 50-110 |
Temperature Display Aaccuracy[oC] | 0.1 |
Temperature Accuracy[oC@55oC] | ±0.3 |
Temperature Uniformity[oC@55oC] | < 0.3 |
Max. Heating/Cooling Rate [°C /sec] | 3 |
Display | 7"800×480 LED |
Touch Operation | Yes |
power-off Protection | Yes |
Power Supply | 100-120V/200-240V, 50/60Hz |
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Other model: YSPCR-10S
Thermo Cycler
Thermal cyclers are essential laboratory equipment in the field of biotechnology cloning, genotyping, mutagenesis, and many other applications. The YSPCR-10G has the precise temperature control system and thermal gradient to easily optimize PCR assays in a single run, makes it possible to quickly and accurately obtain large quantities of DNA needed in molecular biology, clinical diagnosis, criminal investigations and infectious disease research etc.
Features:
• Provides consistent and uniform sealing across an entire block
• Excellent temperature control system
• Fast heating and cooling rate
• Large color touch screen for easy programming
• Quickly and easily identify optimal annealing temperatures by using the programmable temperature gradientSpecificationsSample Capacity | 0.2mL PCR tubes×96,0.2mL×12 PCR 8 strips or 96 well PCR plate |
Heating Temperature Range[oC] | 4-99 |
Lid Temperature Range[oC] | 50-110 |
Temperature Display Aaccuracy[oC] | 0.1 |
Temperature Accuracy[oC@55oC] | ±0.3 |
Temperature Uniformity[oC@55oC] | < 0.3 |
Max. Heating/Cooling Rate [°C /sec] | 3 |
Gradient Temperature | Yes |
Gradient Temperature Range[oC] | 30-99 |
Gradient Range[oC] | 1-42 |
Display | 7"800×480 LCD |
Touch Operation | Yes |
power-off Protection | Yes |
Power Supply | 100-120V/200-240V, 50/60Hz |