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Medical Clinical Real-Time Quantitative PCR Analyzer

China Medical Clinical Real-Time Quantitative PCR Analyzer, Find details about China PCR Analyzer, Real-Time PCR from Medical Clinical Real-Time Quantitative PCR Analyzer

Model NO.
YSPCR-Q96
Detection Device
Pmt
Detection Mode
Time-Resolved Signal Separating Technology
Trademark
YSENMED
Transport Package
Carton
Origin
China
Model NO.
YSPCR-Q96
Detection Device
Pmt
Detection Mode
Time-Resolved Signal Separating Technology
Trademark
YSENMED
Transport Package
Carton
Origin
China
Medical Clinical Real-time Quantitative PCR Analyzer
Medical Clinical Real-Time Quantitative PCR Analyzer
YSPCR-Q96 real-time quantitative PCR system is based on global vision of product design concepts and manufacturing processes. It creatively combines Fresnel lens optical signal acquisition technology, time-resolved signal separation technology and unique temperature control technology. And it reaches inteDNAtional advanced level in sensitivity, multi-color crosstalk, temperature uniformity and accuracy. It supports the application of all common QPCR detection modes.

Up to 6 fluorescence detection channels meet the requirement of multiplex PCR
Effectively reduce multi-color crosstalk and edge effect
New optical scanning detection system--High sensitivity /resolution
Innovative scanning method and time-resolved signal separation technology--High accuracy
Unique edge temperature compensation technology 
Unique design, user-friendly operating software
One-stop process and solution
Classic examples
Swine fever virus detection
QPCR was used to detect the swine fever virus DNA in vitro for clinical diagnosis of suspected diseased pigs.
Kit:Swine fever virus (ASFV) nucleic acid detection kit(PCR probe assay),DNA extraction kit(Spin column method)
Method: 5 mL of blood was extract from a live pig syringe to be examined. DNA was extracted by DNA extraction kit. According to the ASFV detection kit operation method, the extracted DNA, the positive control substance, and the negative control substance is separately added to the PCR reaction solution and the enzyme. Then the mixture is centrifuged and tested on Real-time QPCR system. The reporter and quencher of the TaqMan probe is FAM and TAMRA.
Program:
StepTemperatureTimeCycleEnd point
142ºC20min1No
295ºC10min1No
394ºC15sec40No
55ºC30secYes

Judgment basis:
Positive: Ct≤35,and the amplification curve has a significant exponential growth curve.
Negative:Ct>37,or no significant amplification of the curve.
Recommended retest: 35
Amplification curve:

Data analysis: The Ct value of the positive control is 20, and the negative control product has no obvious amplification curve. The Ct values of the samples 1 and 2 to be tested are 26 and 30 respectively. According to the judgment basis of the kit, the samples to be tested are ASFV positive for classical swine fever virus.
Technical Parameters
Temperature control system
Sample capacity0.2ml PCR tubes×96, 8×12 PCR plate or 96 well plate ×1
Reaction volume10-50 μl
Thermal cycle technologyPeltier
Max. Heating/Cooling rate6.0° C/s
Heating temperature range4 - 100 °C
Temperature accuracy± 0.2°C
Temperature uniformity±0.2ºC @60ºC ,  ±0.3ºC @95ºC
Temperature gradient setting range30-100°C
Temperature gradient difference setting range1 - 36 °C
Detection system
Excitation light source4/6 monochrome high efficiency LEDs
Detection devicePMT
Detection modeTime-resolved signal separating technology
Excitation/detection wavelength range455-650nm/510-715nm
Fluorescent channels4/6 channels
Supported dyeFAM/SYBR Green, VIC/JOE/HEX/TET, ABY/NED/TAMRA/Cy3, JUN, ROX/Texas Red, Mustang Purple, Cy5/LIZ
SensitivitySingle copy gene
Resolution1.33 folds copy number difference can be distinguished in single-plex qPCR
Dynamic range10 orders of magnitude copies


Medical Clinical Real-Time Quantitative PCR Analyzer
Medical Clinical Real-Time Quantitative PCR Analyzer
Medical Clinical Real-Time Quantitative PCR Analyzer

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