HS^TM Kit
#P3082, 5 ml

Contents:

Store at -20°C
For research use only.
In total 6 vials.
 

Description
HS^TM Kit contains HS^TM Taq DNA Polymerase and a premixed, ready-to-use HS^TM Reaction Mix, which contains dNTPs, Mg²⁺ and other components at optimal concentrations for efficient amplification of DNA templates by PCR. To prepare the final PCR, only need to add primers and template DNA. The reaction mix contributes to high specificity by optimizing the system, reducing primer-dimer rate. PCR amplification sensitivity is controllable due to the amount of DNA polymerase is flexible.
HS^TM Taq DNA Polymerase is a thermostable recombinant DNA Polymerase derived from thermophilic bacterium Thermus aquaticus. Its molecular weight is 94 kDa. HS^TM Taq DNA Polymerase can amplify DNA target up to 5 kb. The elongation velocity is 2kb/min. It has 5' to 3' polymerase activity, but lacks of 3' to 5' exonuclease activity that results in 3'-dA overhangs PCR products. All components of the HS^TM Mix are at optimal concentration for efficient amplification. It contributes to highly specific incorporation of primers and template.
Unit Definition
One unit is defined as the amount of the enzyme required to catalyze the incorporation of 10 nmole of dNTPs into an acid-insoluble form in 30 minutes at 70°C using hering sperm DNA as substrate.
Storage Buffer
20mM Tris-HCl (pH8.0), 1mM DTT, 100mM KCl, 3mM MgCl₂, 0.1% NP-40, 0.1% TW 20, 0.2mg/ml BSA, 50% (V/V) Glycerol
Composition of the 2× HS^TM Reaction Mix
2× HS^TM PCR Buffer, 0.4mM dNTPs, 3.2mM MgSO_4, 0.02% bromophenol blue.
HS^TM Reaction Mix is a proprietary formulation optimized for robust performance in PCR.
Applications
• High throughput PCR
• High specificity PCR
• Routine PCR with high reproducibility
• Generation of PCR products for TA cloning
Features
• Convenient: only primers and template DNA are added
 when prepare final PCR
• High yields of PCR products with minimal optimization
• High efficiency: saving your time by simplifying the process   
• Reproducible: lower contamination and pipetting error risk
• High sensitivity: compared to conventional Taq DNA Polymerase
• Flexible: the amount of polymerase is flexible and controllable.