Blood Genomic DNA Extraction Kit

                              For research use only

 

Kit Content

Content	N1121	N1122
Solution RS	80 ml	80 ml x2
Solution DS	15 ml	30 ml
Solution MS	20 ml	40 ml
Proteinase K	1 ml	2 ml
Wash Buffer PS	30 ml	60 ml
Wash Buffer PE	15 ml	30 ml
Elution Buffer TE(10 mM Tris-HCl, pH 8.5)	5 ml	10 ml
Spin Column	50 each	100 each

 

Description

Genomic DNA system uses the silica-gel-membrane technology for simple and fast isolation of Genomic DNA without phenol/chloroform. Homogenization is not necessary since tissues are directly lysed by Proteinase K. The buffer system is optimized to allow selective binding of DNA to the silica-gel membrane. The simple centrifugation protocol completely removes contaminants such as proteins, divalent cations, and secondary metabolites. Pure DNA is then eluted in water or low-salt buffer, ready to use. The kit is suitable for anticoagulated blood. 400 μl whole blood yield 3-10 μg high pure genomic DNA. The purified DNA can be used in common downstream applications such as sequencing, restriction digestion,etc.

Applications

Purified DNA is free from contaminants and enzyme inhibitors, and typically has A₂₆₀/A₂₈₀ ratios between 1.7 and 1.9, and is suitable for applications such as:

• Restriction digestion
• PCR
• Labeling
• Southern hybridisation
• Library construction

Feature. 

• High Efficient - 3-10 ug genomic DNA from 400 ul whole blood
• Safe - no phenol/cholroform extraction step.
• High purity - Purified DNA is ready for downstream application such as PCR, restriction digestion.

Store

store Protein K at -20°C, other reagents can be store at room temperature for up to 1 year. Any precipitate in the Solution DS and solution MS can be re-dissolved by incubating at 37°C before use.

Dongsheng Biotech offers different series of products to help you achieve PCR success. For enzymes, our Taq Polymerase, HS Taq DNA Polymerase, FS Taq DNA Polymerase, Pfu DNA Polymerase and Fusion Pfu DNA Polymerase provide high fidelity, efficient, sensitivity PCR performance. We also have Long Taq DNA Polymerase for long PCR performance.