Basic Info.

Model NO.

P1012

Model NO.

P1012

Product Desciption

Taq DNA Polymerase
#P1012, 500U

 

Concentration: 5U/μl
Contents:

Taq DNA Polymerase	100 μl
10× PCR Buffer (Mg2+ Plus)	1.25 ml
dNTPs (2.5mM each)	1 ml
6× Loading Buffer	1 ml

Store at -20°C
For research use only.

Description
Taq DNA Polymerase is a thermostable recombinant DNA polymerase derived from thermophilic bacterium Thermus aquaticus. Its molecular weight is 94 kDa. Taq DNA Polymerase can amplify DNA target up to 5 kb (simple template). The elongation velocity is 2kb/min (70~75°C). It has 5' to 3' polymerase activity but lacks of 3' to 5' exonuclease activity that results in a 3'-dA overhangs PCR product.
Unit Definition
One unit is defined as the amount of the enzyme required to catalyze the incorporation of 10 nmole of dNTPs into an acid-insoluble form in 30 minutes at 70°C using hering sperm DNA as substrate.
Storage Buffer
20mM Tris-HCl (pH 8.0), 100mM KCl, 3.2mM MgCl_2, 1mM DTT, 0.1% Triton X-100, 0.1% Tween20, 0.2mg/ml BSA, 50% (V/V) glycerol.
10× PCR Buffer with Mg²⁺ 
100mM Tris-HCl (pH 8.8), 500mM KCl, 1% Triton X-100, 16mM MgCl₂.

Applications
• PCR amplification of DNA fragments as long as 5 kb
• DNA labeling
• DNA sequencing
• PCR for cloning

Quality Control
The absence of endodeoxyribonucleases, exodeoxyribonucleases and ribonucleases is confirmed by appropriate quality tests. Functionally tested in amplification of a single-copy gene from human genomic DNA.
Endodeoxyribonuclease Assay
No detectable conversion of covalently closed circular DNA to a nicked DNA was observed after incubation of 10U Taq DNA Polymerase with 1μg pBR322 DNA for 4 hours at 37°C  and 70°C.
Exodeoxyribonuclease Assay
No detectable degradation of lambda DNA-HindIII fragments was observed after incubation of 10U Taq DNA Polymerase with 1μg digested DNA for 4 hours at 37°C and 70°C.
Ribonuclease Assay
0% of the total radioactivity was released into trichloroacetic acid-soluble fraction after incubation of 10U Taq DNA Polymerase with 1μg E.coli [3H]-RNA (40000cpm/μg) for 4 hours at 37°C and 70°C.




Dongsheng Biotech offers different series of products to help you achieve PCR success. For enzymes, our Taq Polymerase, HS Taq DNA Polymerase, FS Taq DNA Polymerase, Pfu DNA Polymerase and Fusion Pfu DNA Polymerase provide high fidelity, efficient, sensitivity PCR performance. We also have Long Taq DNA Polymerase for long PCR performance.