Quick Yeast Genomic DNA Ectraction Kit

                  For research use only

Quick Yeast Genomic DNA Extraction Kit	Catalog#	Size
	N1161	50 preps
	N1162	100 preps

Kit Contents

Content	N1151	N1152
Solution DS	15 ml	30 ml
Solution MS	20 ml	40 ml
Proteinase K	1 ml	2 ml
Wash Buffer PS	30 ml	60 ml
Wash Buffer PE	15 ml	30 ml
Eluent Buffer TE	5 ml	10 ml
Spin Column	50 each	100 each

 

Description
The Yeast Genomic DNA Purification Kit provides a simple and rapid method for high quality genomic DNA purification from yeast. The Yeast Genomic DNA system uses the silica-gel-membrane technology for simple and fast isolation of Genomic DNA without phenol/chloroform. Homogenization is not necessary since tissues are directly lysed by Proteinase K. The buffer system is optimized to allow selective binding of DNA to the silica-gel membrane. The simple centrifugation protocol completely removes contaminants such as proteins, divalent cations, and secondary metabolites. Pure DNA is then eluted in water or low-salt buffer, ready to use. The typical yield of genomic DNA is 3-25 µg from 1 - 5 x 10⁷ yeast cells. The purified high molecular weight genomic DNA is suitable for direct use in all common molecular biology applications: PCR, restriction digestion, cloning, DNA sequencing and Southern blot analysis.

Features
Efficient - 3-35 ug of genomic DNA from 1 - 5 x 10⁷ yeast cells.
Fast - procedure takes only 1 h.
Safe - no phenol/chloroform extraction step.
High purity - Purified DNA is ready for downstream application such as PCR, restriction digestion.

Downstream Applications
Purified DNA is free from contaminants and enzyme inhibitors, and typically has A₂₆₀/A₂₈₀ ratios between 1.7 and 1.9, and is suitable for applications such as:

Restriction digestion

PCR

Labeling

Library construction

Store
store Protein K at -20°C, other reagents can be store at room temperature for up to 1 year. Any precipitate in the Solution DS and solution MS can be re-dissolved by incubating at 37°C before use.
 

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