Hoechst 33342 (Bisbenzimide H 33342, HOE 33342) The molecular formula is C₂₇H₂₈N₆O·3HCl, the molecular weight is 561.93, and the CAS number is 23491-52-3, which is a blue fluorescent dye that penetrates the cell membrane to the nucleus DNA. The staining fluid is weak in the solution, but the fluorescence reinforcement is bonded to the DNA small groove in the AT sequence enriched region, and thus is used for nuclear staining, or conventional DNA staining.

This product is a solution, and the Hoechst 33342 concentration is 1 mg / ml for staining, and the commonly used work concentration is recommended from 0.5 to 10 μg / ml. The maximum excitation wavelength of Hoechst 33342 is 346 nm, the maximum emission wavelength is 460 nm; after the binding with the double-stranded DNA, the maximum excitation wavelength is 350 nm, the maximum emission wavelength is 461 nm. The nucleus can be used for a fixed or non-fixed cell or tissue nucleus Mark, and can be tested by fluorescent microscope or Flow cytometer etc.