Product Introduction

RPMI in RPMI-1640 is the abbreviation of Roswell Park Memorial Institute, which stands for Roswell Park Memorial Institute. RPMI-1640 is a cell culture medium developed by the institute. This medium was originally designed specifically for lymphocyte culture, and has been widely used in the culture of various normal cells and cancer cells, especially the culture of suspension cells, and is one of the most widely used culture media.

This product contains amino acids, vitamins, inorganic salts and other ingredients required for cell culture. It does not contain protein or growth factors. It is necessary to add 5-10% serum or serum-free additives according to the cell type.

This product is sterilized by filtration through a 0.1 μm filter membrane, pH 7.0-7.4, containing 11.1 mM D-glucose, supplemented with 2.05 mM L-glutamine, containing penol red indicator, containing 25 mM HEPES buffer system, without sodium BT .

HEPES is an excellent biological buffer, which has no toxic effect on cells. The medium with HEPES can maintain a constant pH range for a long time.


Storage and transportation

Transport with wet ice; store at 2~8ºC, valid for 12 months.


Precautions

1. This product is filtered and sterilized, and aseptic operation should be paid attention to when using it to avoid contamination;

2. In order to maintain the best results, please do not freeze-thaw.