Lincomycin acts on the 50S subunit of the ribosome of sensitive bacteria to prevent the elongation of the peptide chain, thereby inhibiting the protein synthesis of bacterial cells. Lincomycin is generally a bacteriostatic agent, but at high concentrations, it also has a bactericidal effect on highly sensitive bacteria

 It is mainly used for the treatment of infections caused by Gram-positive bacteria in particular the various penicillin-resistant Gram-positive bacteria , the poultry chronic respiratory disease caused by Mycoplasma, swine enzootic pneumonia, anaerobic infections such as chicken necrotizing enterocolitis.

It can also be used for the treatment of treponema dysentery, toxoplasmosis and actinomycosis of dogs and cats.