The pSWE-Topo Zero Cloning Kit produced by our company is a vector for screening positive recombinants through the expression of suicide genes and is modified from the pUC19 vector. When the target gene fragment is not connected to the vector, the suicide gene is successfully and correctly expressed, and the cells containing the vector cannot grow; when the target gene fragment is successfully connected to the vector, the suicide gene will not be expressed correctly, and the cells containing the vector can grow normally. This vector is also called "Zero" background vector. At the same time, our company's pSWE-Topo Zero Cloning Kit uses a new vector ligation method, no additional ligase is needed to connect the fragment to the vector, only the target gene fragment needs to be added to the vector at room temperature (22ºC-37 ºC) The conversion operation can be carried out after reacting for 5 minutes. pSWE-Topo Zero Cloning Kit is compatible with TA cloning and blunt-end cloning.

Features:

Easy to use, compatible with TA cloning and blunt-end cloning; no additional enzyme reaction is required, the target gene fragment is added to the system, and normal transformation can be carried out within 5 minutes at room temperature; the positive rate can reach 95%; short and long fragments Applicable for cloning; selection marker is ampicillin; sequencing primers: M13 Forward Primer, M13 Reverse Primer.

Component:

1. Store the product in an ice box or on an ice bath before use, and store it at -20ºC immediately after use.
2. Escherichia coli strains DB3.1, CCDB Survival, Stable, JM109, XL1-Blue and XL10-Gold can tolerate CCDB and can be used to reproduce or preserve plasmids containing CCDB. Therefore, PSWE-Topo Zero Vector is not applicable for filtering. DH5α, TOP10, TG1 and other competent Escherichia coli cells that are not tolerant to CCDB commonly used in other laboratories are applicable.
3. For your safety and health, please wear lab coat and disposable gloves for operation.

This product is only for scientific research purposes, not for clinical diagnosis!