T4 DNA Ligase for Terminal Double Stranded DNA Catalysis

China T4 DNA Ligase for Terminal Double Stranded DNA Catalysis, Find details about China DNA Ligase, Sticky End DNA Ligse from T4 DNA Ligase for Terminal Double Stranded DNA Catalysis

Basic Info.

Model NO.

G3340-100

Storage Conditions

at -20ºC

Transportation Condition

Wet Ice Transportation

Validity

12 Months

Trademark

Servicebio

Transport Package

Standard Package

Specification

500U; 250U

Origin

China

HS Code

3204901000

Model NO.

G3340-100

Storage Conditions

at -20ºC

Transportation Condition

Wet Ice Transportation

Validity

12 Months

Trademark

Servicebio

Transport Package

Standard Package

Specification

500U; 250U

Origin

China

HS Code

3204901000

Product Desciption

T4 DNA Ligase for Terminal Double Stranded DNA Catalysis

T4 DNA Ligase can catalyze the bonding of sticky or blunt-ended double-stranded DNA or RNA between the 5'-P end and the 3'-OH end with a phosphodiester bond. At the same time, the enzyme can also repair single-stranded nicks in double-stranded DNA, RNA, and DNA/RNA hybrid chains.

Activity definition: One unit of enzyme can catalyze 1 nmol of 32P-labeled pyrophosphate into ATP through displacement reaction within 20 minutes at 37°C (one unit is equal to about 200 sticky-end attachment units).

T4 DNA Ligase Storage buffer:20 mM Tris-HCl (pH 7.5), 50 mM KCl, 1 mM DTT, 50% (v/v) glycerol.

5×T4 DNA Ligase Buffer:250 mM Tris-HCl (pH 7.6), 50 mM MgCl₂, 5 mM ATP, 5 mM DTT, Enhancer.

Component Number	Component	G3340-50	G3340-100
G3340-1	T4 DNA Ligase	250 U (50 μL)	500 U (100 μL)
G3340-2	5×T4 DNA Ligase Buffer	1 mL	2×1 mL
User Manual		1pc