Product Introduction

Ham's F-12K medium is a Kaighn's modified version of Ham's F-12 nutrient mixture. It was originally designed for culturing primary human hepatocytes and differentiated rat and chicken cells under low serum conditions. Ham's F-12K increases the concentration of amino acids and sodium pyruvate on the basis of the original Ham's F-12, reduces the content of glucose, and modifies the composition and content of inorganic salts. Compared with other media, Ham's F-12K contains many unique ingredients, such as putrescine, thymine, hypoxanthine, zinc, etc., and the content of sodium pyruvate is also higher.

This product contains amino acids, vitamins, inorganic salts and other ingredients required for cell culture, and does not contain protein or growth factors. Serum or serum-free additives must be added according to the cell type.

This product is sterilized by filtration through a 0.1 μm filter membrane, pH 7.0-7.4, containing 7.0 mM D-glucose, adding 2.0 mM L-glutamine and 2.0 mM sodium pyruvate, containing phenl red indicator, without HEPES buffer system.

Storage and transportation

Transport at room temperature; store at 2-8°C, valid for 12 months.


Precautions

1. This product has been filtered and sterilized. When using it, you should pay attention to aseptic operation to avoid contamination;

2. In order to maintain the best results, please do not freeze-thaw.