SDS-Page Electrophoresis Gel Preparation Kit

China SDS-Page Electrophoresis Gel Preparation Kit, Find details about China Gel Ultra-Fast Preparation Kit, SDS-Page Gel Preparation Kit from SDS-Page Electrophoresis Gel Preparation Kit

Basic Info.

Model NO.

G2003-50T

Application

Laboratory/University/Hospital/Medical Center Usag

Trademark

Servicebio

Transport Package

Carton

Specification

Polypropylene

Origin

China

Model NO.

G2003-50T

Application

Laboratory/University/Hospital/Medical Center Usag

Trademark

Servicebio

Transport Package

Carton

Specification

Polypropylene

Origin

China

Product Desciption

Product Introduction
The SDS-PAGE gel preparation kit of this product contains all the reagents required for gel preparation. The user only needs to prepare a small amount of pure water and glue making equipment (glue base, glass plate, comb, etc.), and operate according to the instructions. Required gel. This kit can prepare at least 50 pieces of regular size gels.
Storage and transportation
Transport in wet ice; store at 4°C and protect from light. The validity period is 12 months.

Component Number	Component	G2003-50T
G2003-1	1.5 M Tris-HCl(pH 8.8)	100 mL
G2003-2	30% Acrylamide(29:1)	100 mL
G2003-3	1.0 M Tris-HCl(pH 6.8)	20 mL
G2003-4	10% SDS	5 mL
G2003-5	AP(Ammonium persulfate)	3×180 mg
G2003-6	TEMED	500 μL
manual		1

Instructions
1. According to the molecular weight of the target protein and the selected electrophoresis buffer, select the appropriate concentration of SDS-PAGE lower layer gel, refer to the following table:1.

SDS-PAGE Separating glue concentration	Best separation range(kDa) (Tris-Glycine Electrophoresis buffer,G2018)	Best separation range(kDa) (SWEFast high resolution electrophoresis buffer,G2081)
6%	50-300	15-300
8%	30-130	10-250
10%	20-100	5-150
12%	10-60	3-100
15%	<40	<60

2. According to the molecular weight of the target protein, select the concentration of the separation gel. Take the commonly used 8.3 cm×7.3 cm gel plate (single piece) as an example. You can refer to the following table to prepare the gel solution:

Separating glue concentration(%)	6%			8%			10%			12%			15%			18%			20%
Glass plate thickness	0.75 mm	1.0 mm	1.5 mm	0.75 mm	1.0 mm	1.5 mm	0.75 mm	1.0 mm	1.5 mm	0.75 mm	1.0 mm	1.5 mm	0.75 mm	1.0 mm	1.5 mm	0.75 mm	1.0 mm	1.5 mm	0.75 mm	1.0 mm	1.5 mm
Total volume of glue solution required*(mL)	4.0	6.0	8.0	4.0	6.0	8.0	4.0	6.0	8.0	4.0	6.0	8.0	4.0	6.0	8.0	4.0	6.0	8.0	4.0	6.0	8.0
H2O(mL)	2.12	3.18	4.24	1.85	2.78	3.7	1.59	2.38	3.17	1.32	1.98	2.64	0.92	1.38	1.84	0.52	0.78	1.04	0.25	0.38	0.51
30% Acrylamide(29:1)(mL)	0.8	1.2	1.6	1.07	1.6	2.14	1.33	2.0	2.67	1.6	2.4	3.2	2.0	3.0	4.0	2.4	3.6	4.8	2.67	4.0	5.3
1.5 M Tris-HCl(pH 8.8)(mL)	1.0	1.5	2.0	1.0	1.5	2.0	1.0	1.5	2.0	1.0	1.5	2.0	1.0	1.5	2.0	1.0	1.5	2.0	1.0	1.5	2.0
10% SDS(μL)	40	60	80	40	60	80	40	60	80	40	60	80	40	60	80	40	60	80	40	60	80
10% AP(Ammonium persulfate)μL	40	60	80	40	60	80	40	60	80	40	60	80	40	60	80	40	60	80	40	60	80
TEMED(μL)	2.0	3.0	4.0	2.0	3.0	4.0	2.0	3.0	4.0	2.0	3.0	4.0	2.0	3.0	4.0	2.0	3.0	4.0	2.0	3.0	4.0

^*The total volume of glue making solution does not include the volume of TEMED.

3. For the preparation of concentrated glue, please refer to the following table:

Concentration of concentrated glue(%)	5%
H₂O(mL)	1.93	2.95	3.86	5.79
30% Acrylamide(29:1)(mL)	0.5	0.75	1.0	1.5
1.0M Tris-HCl(pH 6.8)(mL)	0.5	0.75	1.0	1.5
10% SDS(μL)	40	60	80	120
10% AP(Ammonium persulfate)(μL)	30	45	60	90
TEMED(μL)	4	6	8	12
total volume^*(mL)	3	4.5	6.0	9.0

^*The total volume does not include the TEMED volume.

4. Recommended electrophoresis conditions:
a. Use SWE fast high-resolution electrophoresis buffer (G2081) for electrophoresis: 200-250 V constant voltage, 25-35 minutes to complete electrophoresis;
b. Use Tris-Glycine electrophoresis buffer (G2018) for electrophoresis: set the voltage of the upper gel to 90 V, and electrophoresis for about 30 minutes (marker enters the separation gel); adjust the voltage of the lower gel to 150-180 V, about 60-90 min (can be Adjust according to the actual situation).
Precautions
1. All reagents need to be warmed to room temperature in advance before use.
2. AP (ammonium persulfate) is prepared with pure water to a concentration of 10%, that is, 1800 μL of pure water is added to each 180 mg AP (ammonium persulfate) tube to dissolve. The AP prepared as a solution is recommended to be stored in small aliquots at -20°C, with a validity period of 3 months; if stored at 4°C, the validity period is 1 week.
3. It is recommended to use Tricine gel for protein separation less than 10 kDa. Acrylamide gel may not be sufficient for separation.
4. Temperature has an effect on the setting time of the glue. Generally, the lower the temperature, the longer the setting time; the higher the temperature, the faster the gel.
5. The gel needs sufficient setting time. It is recommended to make the gel and let it stand fully to ensure that the gel is complete.
6. When the temperature is low, the 10% SDS solution will crystallize out, and it can be used after reheating and melting at 37°C.
7. If you need quick gel preparation reagents, you can purchase other products of our company, such as SDS-PAGE rapid gel preparation kit (G2037), or ultra-fast color gel preparation kit series (G2041/G2042/G2043/G2044/G2045) , G2060/G2061/G2062/G2063/G2064), and high-resolution ultra-fast color gel preparation kit series (G2066/G2067/G2068, G2071/G2072/G2073), which can meet different experimental needs.
8. For your safety and health, please wear lab coats and disposable gloves for operation.
SDS-Page Electrophoresis Gel Preparation Kit