Product Description
Product Name Polyadenylic Acid, Poly (A), Carrier RNA
Cat. No. & Specifications C12100,1g/10g/100g
CAS: 26763-19-9, E.C. 3.4.21.64
Synonyms: Peptidase K, Endoproteinase K, Endopeptidase K
Introduction
Poly(A) is a suitable carrier for quantitative precipitation of DNA and RNA, especially to improve recovery of low amounts of nucleic acid or of short fragments <200 bp.
Application
1. Appearance (Form) : Lyophilized Powder, White to White/ Yellow Cast
2. Solubility (Solvent): RNase Free Water
3. Purity: > 95.00 %
4. UV (Lambda max): 255~258 nm
5. A260/A230 UV Absorption: 4.5~5.0 (0.5mg/ml)
6. A260/A280 UV Absorption: 2.8~3.1 (0.5mg/ml)
7. Molecular weight: 100-500KD
8. Ribonucleases (fluorescence polarization): Negative
Ordering information Cat.No. | CA-310 | CA-1 |
Poly A, Lyophilizate | 310 ug/Tube | 1 g |
Special Quality Inspection1. Size ( Agarose gel electrophoresis after hybrid with Oligo(dT): 100-5000nt, mostly at 500~3000nt.
2. Inhibitors: Do not inhibit RT-PCR (addition of 2ul of 1ug/ul Poly A to 25ul Real Time RT-PCR Reaction).
3.Function: Viral RNA were isolated from 0.2ml Plasma (Contain 100IU/ml HCV) by using HiPure Viral DNA/RNA Kit. Addtion of 0ug or 4ug Poly A as Carrier RNA to the lysis Buffer. Additon of Carrier RNA significantly reduced by 1-3 CT Value compared to the control group(no addition of Poly A).
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