China Factory Direct Sale Lab Comsumables Hlb Solid Phase Extraction Column, Find details about China Solid Phase Extraction Column, Hlb Spe Column from Factory Direct Sale Lab Comsumables Hlb Solid Phase Extraction Column
Details
Copure® HLB sorbent is composed of monodisperse N-vinylpyrrolidone-divinylbenzene copolymer resin particles, with specific mixture of hydrophilic hydrophobic groups, allowing for retention for a wide range of compounds with very high capacity.
HLB can be used as a general-purpose sorbent, especially for extracting analytes from complicated samples such as blood and urine.
SPE Cartridge: Biocomma HLB, 200mg/3mL
System: Waters alliance 2690
Column: Welch Ultimate XB-C18 (4.6*250mm)
Mobile phase A: acetronile
Mobile phase B: water containing 0.1% formic acid
Flowrate: 1 mL/min
Column temperature: room temperature
Injection volume: 20 μL
Detector: UV at 220 nm
Gradient:
Step | Time (min) | A% | B% |
---|---|---|---|
1 | 0 | 9 | 91 |
2 | 11 | 9 | 91 |
3 | 20 | 29 | 71 |
4 | 25 | 37 | 63 |
5 | 26 | 100 | 0 |
6 | 30 | 100 | 0 |
7 | 31 | 9 | 91 |
8 | 36 | 9 | 91 |
Results:
Retention Time (min)
Peak | Retention time (min) | Analyte | Recovery |
---|---|---|---|
1 | 21.508 | Enoxacin | 92.8% |
2 | 22.820 | Pefloxacin | 82.4% |
3 | 24.201 | Danofloxacin | 93.8% |
4 | 24.754 | Enrofloxacin | 81.2% |
The results suggest that recoveries of > 80% are obtained by using Biocomma HLB cartridges to extract the four Quinolones in foods, meeting the criteria of China official method GB/T 21312-2007.
Product No. | Description | Format | Qty. |
---|---|---|---|
COHLB330 | Copure® HLB SPE Cartridges | 30mg/3mL | 50/Box |