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Glycine Silver Staining Kit for Nerve Tissue Pathology Stain
Glycine Silver Staining Kit for Nerve Tissue Pathology Stain

Glycine Silver Staining Kit for Nerve Tissue Pathology Stain

China Glycine Silver Staining Kit for Nerve Tissue Pathology Stain, Find details about China Glycine Silver, Pathology Stain from Glycine Silver Staining Kit for Nerve Tissue Pathology Stain

Model NO.
G1052-500T
Habit Appellation
Special Reagent
Application
Industry, Scientific Research
Property
Biochemical Reagent
Trademark
Servicebio
Specification
4× 200 mL
Origin
China
Model NO.
G1052-500T
Habit Appellation
Special Reagent
Application
Industry, Scientific Research
Property
Biochemical Reagent
Trademark
Servicebio
Specification
4× 200 mL
Origin
China
Principle
Axon in nerve fiber is argyrophilic. In this silver staining method, tissues are firstly treated with silver nitrte, then react with silver-amonia solution. Silver ion aggregate in axon due to the argyrophilic. Finally display the silver ion by reducing it to black elemental silver using reductant. This kit applies to paraffin sections and frozen-slides, usually used in studying neuron fibril pathological changes in Alzheimer's disease and non-Alzheimer's disease, detecting the degree and extent of degeneration of peripheral nerve in leprosy, and differentiating neurofibroma (positive) from neurinoma (negative).  

Package content
 
Code Name Size
G1052-1Silver nitrte solution (avoiding light)50 ml
G1052-2Concentrated amonia aqua solution5 ml
G1052-3Diluted amonia aqua solution50 ml
G1052-4Developing stock solution100 ul
G1052-5Sodium thiosulfate solution50 ml

Preservation
Valid in 3 months when stored at 4ºC and kept in dark condition.



Instructions
  1. Deparaffinize and rehydrate to distilled water. Wash in distilled water twice.
  2. Marked the objective area with PAP PEN, leaving spaces between different areas. Cover slides with silver nitrte solution and place in a capped black wet box device, incubate for 20 min. Wash with distilled water.
  3. Silver-amonia solution (prepare according to need): take a certain amount of silver nitrte solution, adding concentrated amonia aqua dropwise with stirring until dark-brown precipitation appear. Then add concentrated amonia aqua continually until the precipitation disappear. This silver-ammonia solution can be stored in dark condition for several days.
  4. Cover slides with silver-amonia solution for 15 min.
  5. Rinse in diluted amonia aqua solution and immerse slides in the same solution. (Prepare developing solution meanwhile).
  6. Dilute developing stock solution in 1:1500 with silver-amonia solution (Prepare before use), ensure it is clarified. If any turbidity appears, it is not suitable for use.
  7. Developing slides by adding developing solution, wait for 8-15 min and then manage the appropriate coloration extent by observing in microscopy. When neuron fibers are black in a golden yellow background, stop developing by wash in distilled water. (If developing is inadequate, apply more developing solution).
  8. Rinse in sodium thiosulfate solution for 2 min (remove residual unreacted silver ion )
  9. Wash in running tap water for 5 min.
  10. Dehydrate in gradient alcohol solution of 75%, 85%, 95%, 2 changes of absolute alcohol, respectively, and several minutes each. Clear in xylene and mount. (Be sure to mount when sections are still moist before xylene evaporate completely, or else black crystal substance would appear.)
Results
Neuron axon, neuron fiber, neurofibrillary tangles, senile plaques and dendrite are shown black in gold yellow background.

Notes
  1. Be sure to wash slides with distilled water. Tap water will result in precipitation.
  2. The developing solution are stable for a long time and valid in a year.
  3. Manage the proper developing time. Inadequate developing result in yellow axon, while over-developing may result in black precipitations and collagen fibers stained with brown-black. 
  4. For your safe and health, please wear the lab coat and wear a disposable glove during operation.

Examples
Glycine Silver Staining Kit for Nerve Tissue Pathology Stain
 Glycine Silver Staining Kit for Nerve Tissue Pathology Stain

A Peritoneal tissue, neuron fibers are black in colorless background.
B Central nerve system, neuron fibers are black in gold-yellow background
Glycine Silver Staining Kit for Nerve Tissue Pathology StainGlycine Silver Staining Kit for Nerve Tissue Pathology StainGlycine Silver Staining Kit for Nerve Tissue Pathology StainGlycine Silver Staining Kit for Nerve Tissue Pathology StainGlycine Silver Staining Kit for Nerve Tissue Pathology Stain

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