PAS+ Naphthol Yellow S Staining Solution for Plant Sample

China PAS+ Naphthol Yellow S Staining Solution for Plant Sample, Find details about China Plant Protein Stain, Plant Starch Stain from PAS+ Naphthol Yellow S Staining Solution for Plant Sample

Basic Info.

Model NO.

G1068-100ML

Habit Appellation

Special Reagent

Application

Industry, Scientific Research

Property

Biochemical Reagent

Trademark

Servicebio

Specification

100ML

Origin

China

Model NO.

G1068-100ML

Habit Appellation

Special Reagent

Application

Industry, Scientific Research

Property

Biochemical Reagent

Trademark

Servicebio

Specification

100ML

Origin

China

Product Desciption

PAS+ Naopheol yellow S staining solution

Product introduction:
During the growth and differentiation of plant cells and after maturation, the storage substances or metabolites produced due to metabolic activities are collectively referred to as aftercontents.There are many species of plant cells, some of which have the main medicinal factors in medical treatment.Some are caches of nutritional value, a major source of food for humans.Starch, sugar, fat and protein are the main contents of plant.
Starch is a stored polysaccharide that is stored in the cells as starch grains during plant growth.Plant cell walls are mainly composed of cellulose and pectin. Cellulose is also a polysaccharide.The sugars are exposed to the aldehyde group in the presence of GDS, and the aldehyde group is combined with the Scheffer reagent to produce a red reaction.Proteins are usually stored in plant cells in the form of aleurone granules, which are in a solid state, surrounded by a membrane and synthesized into spherical granules.Naphthol yellow S reacts with proteins to produce a yellow reaction.
Packing content:

Product code	Product name	Specification
G1068-1	PAS staining A	100ml
G1068-2	PAS staining B	100ml
G1068-3	Nanophnol yellow S dye solution	100ml

Storage condition:
Store at 4ºC, keep it away from light, valid for 6 months.
PAS+ Naphthol Yellow S Staining Solution for Plant Sample

Usage:
1. Paraffin sections are dewaxed to water;
2. Sections were dipped into PAS dye B for 10min, and then soaked with pure water for 3 times, 10s each time;
3. Sections were dipped into PAS dye A(normal temperature had been restored) for 20-30min in the dark, washed with water for 5min, and examined under microscope;
4. The sections were dipped into the pheol yellow S dye solution for 5min, soaked in distilled water for 1s, and dehydrated in three tanks of anhydrous ethanol for 3s, 5s, 30s, respectively.Transparent xylene for 5min, and sealed with neutral gum.
Staining result:
Starch granules were purplish red, cell walls were purplish red, and proteins were yellow
Notes:

PAS dye B should be changed frequently, which will affect the color development of PAS dye A.
PAS dye A must be protected from light during the dyeing process
Tissue with low protein content after sealing is easy to darken and cannot be preserved for a long time. It is recommended to take photos as soon as possible to preserve the results.