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IP Lysis Buffer for Protein Samples Lab Biology Experiments Reagent

China IP Lysis Buffer for Protein Samples Lab Biology Experiments Reagent, Find details about China IP Lysis Buffer, Western Blot Lysis Buffer from IP Lysis Buffer for Protein Samples Lab Biology Experiments Reagent

Model NO.
G2038-100ML
Habit Appellation
Special Reagent
Application
Industry, Scientific Research
Property
Biochemical Reagent
Trademark
Servicebio
Specification
100ML
Origin
China
Model NO.
G2038-100ML
Habit Appellation
Special Reagent
Application
Industry, Scientific Research
Property
Biochemical Reagent
Trademark
Servicebio
Specification
100ML
Origin
China
Product introduction:
IP lysis Buffer is a lysate that lyses cells or tissues under non-denaturing conditions, which can retain the interaction between protein and protein or protein and nucleic acid in the cell. The samples lysed in this lysate are mainly used in special experiments such as immunoprecipitation IP(ImmunolPrecipitation),co-immunoprecipitation(co-IP), ChIP (ChromatinImmunopre-cipitation). the mainly components are Tris-Hcl, NaCl, EDTA-Na2, NP-40, Sodium deoxycholate.

Package Contents:
Product Item No.Product NameSpecification
G2038IP Lysis Buffer 100 mL

Storage conditions: Store at 4ºC, valid for 12 months.

Instructions:

For tissue samples:

1. The tissue pieces are washed with cold PBS to remove blood stains. Cut into small pieces and place in a homogenizer.

2. Add 10 times the volume of the tissue to this reagent (add protease inhibitor within a few minutes before use) and homogenize thoroughly with a high-speed tissue grinder.

3. Transfer the homogenate to a 1.5 mL centrifuge tube and shake. In the ice bath for 30 minutes, pipette repeatedly to ensure complete cell lysis.
 
  1. Centrifuge at12000g for 5 min, and collect the supernatant, which is the total protein solution.

For cultured cell samples:

1. For adherent cells:

A. Wash the cells 2-3 times with PBS. Thoroughly absorb the remaining liquid one last time.

B. Add an appropriate volume of this reagent (add protease inhibitor within a few minutes before use) in the culture plate and bottle for 3-5 min. During this period, the culture plate and bottle were shaken repeatedly to make the reagents fully contact the cells.

C. Scrape the cells and reagents with a cell scraper and collect them in a 1.5 mL centrifuge tube.

2. For suspension cells:

Collect the cells by centrifugation, add about 250 uL of this reagent (add protease inhibitor within a few minutes before use) per 6-well plate cells, and shake.

Ice bath for 30 minutes, during which time, pipette with a 200uL pipette several times every 10 minutes to ensure that the cells are completely lysed.

3.Centrifuge at 12000g for 5 min, and collect the supernatant, which is the total protein.

Precautions:
For tissue samples:
Approximately 1mL of this reagent is added to lyse every 50mg tissue. For tissues such as cartilage and skin, the dosage of reagents can be appropriately reduced to increase the protein concentration.

For cultured cell samples:

1. Under normal cell density, add about 250uL of lysate to each 6-well plate cell.

2. It may appear thicker during cracking. It can be pipetted repeatedly until it is liquid. If it has been thicker, you can use a vortex to shake or add an appropriate amount of lysate.
3.This reagent is harmful to the human body, please protect it appropriately.
IP Lysis Buffer for Protein Samples Lab Biology Experiments Reagent
IP Lysis Buffer for Protein Samples Lab Biology Experiments ReagentIP Lysis Buffer for Protein Samples Lab Biology Experiments ReagentIP Lysis Buffer for Protein Samples Lab Biology Experiments ReagentIP Lysis Buffer for Protein Samples Lab Biology Experiments ReagentIP Lysis Buffer for Protein Samples Lab Biology Experiments ReagentIP Lysis Buffer for Protein Samples Lab Biology Experiments Reagent

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