Viral RNA/DNA Miniprep Kit (Magnetic Beads)
For Hanks or PBS Buffer
Catalog No.: V4009 Size: 200 preps
Description
Based on the method of magnetic bead separation and purification, the Viral RNA/DNA Miniprep Kit (Magnetic Beads) For Hanks or PBS Buffer is suitable for the purification of high quality viral nucleic acid from throat swab and nasal swab protected in virus preservation solution of non-inactivation type, such as Hanks Balanced Salt Solution (HBSS) and PBS solution. The sensitivity of nucleic acid detection can be improved by increasing the sample amount for nucleid acid extraction.
The purification system uses superparamagnetic nano-magnetic particles as the matrix, which can adsorb nucleic acids specifically through hydrogen bonds and static electricity under the condition of high concentration of leachate, while proteins and other non-specific impurities are removed by washing. Finally the nucleic acid are eluted with low salt buffer or RNase-free ddH2O. The purified nucleic acid can be used for various routine operations, including RT-PCR, RT-qPCR, fluorescence quantitative PCR (qPCR) and other downstream experiments.
Main componentsThe kit consists of the following components:The name of the reagent | Amount | Component description |
Buffer MVH | 45 ml | Provide environment for lysing and binding to the magnetic beads |
Buffer MW | 20 ml × 3 | Remove residual proteins and other impurities |
Mag Beads | 4.5 ml | Adsorb viral nucleic acid |
DEPC-Water | 12 ml | DEPC - treated water, RNase - free |
DS Carrier | 450 µl | Capture trace nucleic acid |
Proteinase K | 1 ml × 4 | Lyses proteins bound to nucleic acids |
Storage conditionsStore DS Carrier and Proteinase K at -20°C, Mag Beads and DEPC-Water at 2-8°C, others at room temperature (RT, 15-25°C), and transport at RT.