Trazol / Rna Extraction Reagent, 20ml R1021

China Trazol / Rna Extraction Reagent, 20ml R1021, Find details about China Extraction, Silica-Based Membrane Technology from Trazol / Rna Extraction Reagent, 20ml R1021

Model NO.
R1021
Model NO.
R1021
Component
R1021
R1022
TRAzol Reagent
20 ml
100 ml

TRAzol Reagent

TRAzol Reagent is a ready-to-use reagent for the isolation of total RNA from cells and tissues. The reagent, a mono-phasic solution of phenol and guanidine isothiocyanate, is an improvement to the single-step RNA isolation method. During sample homogenization or lysis, TRAzol Reagent maintains the integrity of the RNA, while disrupting cells and dissolving cell components. Addition of chloroform followed by centrifugation, separates the solution into an aqueous phase and an organic phase. RNA remains exclusively in the aqueous phase. After transfer of the aqueous phase, the RNA is recovered by precipitation with isopropyl alcohol. After removal of the aqueous phase, the DNA and proteins in the sample can be recovered by sequential precipitation. Precipitation with ethanol yields DNA from the interphase, and an additional precipitation with isopropyl alcohol yields proteins from the organic phase. Copurification of the DNA may be useful for normalizing RNA yields from sample to sample.This technique performs well with small quantities of tissue (50-100 mg) and cells (5 × 106), and large quantities of tissue (≥1 g) and cells (>107) of human, animal, plant, or bacterial origin.The simplicity of the TRAzol Reagent method allows simultaneous processing of a large number of samples. The entire procedure can be completed in one hour. Total RNA isolated by TRAzol Reagent is free of protein and DNA contamination. 

Trazol / Rna Extraction Reagent, 20ml R1021

Features 

•Stable yield
•Reliable performance of high-quality purified total RNA in downstream applications

 

Trazol / Rna Extraction Reagent, 20ml R1021

 

Trazol / Rna Extraction Reagent, 20ml R1021

Dongsheng Biotech offers different series of products to help you achieve PCR success. For enzymes, our Taq Polymerase, HS Taq DNA Polymerase, FS Taq DNA Polymerase, Pfu DNA Polymerase and Fusion Pfu DNA Polymerase provide high fidelity, efficient, sensitivity PCR performance. We also have Long Taq DNA Polymerase for long PCR performance.

Trazol / Rna Extraction Reagent, 20ml R1021